Cross-talk between gama-aminobutyric acid and calcium ion regulates lipid biosynthesis in Monoraphidium sp. QLY-1 in response to combined treatment of fulvic acid and salinity stress. (November 2020)

• Record Type: Journal Article
• Authors: Li, Ximing; Zhang, Xinnan; Zhao, Yongteng; Yu, Xuya
• Is Part Of: Bioresource technology. Volume 315(2020)
• Publication Date: 2020-11
• Subjects: Gama-aminobutyric acid -- Calcium ion -- Lipid -- Microalgae -- Fulvic acid -- Salinity stress; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2020.123833
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: GABA treatment enhanced lipid biosynthesis under salinity stress with fulvic acid. GABA decreased ROS levels of QLY-1 under salinity stress with fulvic acid. GABA regulated calcium signalling in QLY-1 under salinity stress with fulvic acid. Calcium promoted lipid synthesis by regulating GABA shunt. Abstract: In this study, the cross-talk between gama-aminobutyric acid (GABA) and calcium ion (Ca 2+ ) signalling in the regulation of lipid production and cell growth in microalgae under fulvic acid and salinity stress (FA-salinity treatment) was investigated. GABA enhanced the lipid content and lipid productivity rate considerably, which were 1.27 and 1.29 times higher than those of the control, respectively. The levels of biosynthetic gene transcription, GSH, Ca 2+ and cellular GABA were promoted by GABA addition, but decreased the ROS levels. Furthermore, the application of Ca 2+ also increased lipid synthesis by regulating ROS and GABA signalling and lipogenesis-related genes. These results indicated that cytosolic GABA and Ca 2+ levels exert crucial cross-talk in the modulation of cell growth and lipid accumulation induced by FA-salinity treatment. Collectively, this study demonstrated the beneficial effects caused by induction of the combination of chemical compounds on lipid production and provided new insights into lipid synthesis in microalgae.