Efficient conversion of chitin-derived carbon sources into microbial lipid by the oleaginous yeast Cutaneotrichosporon oleaginosum. (November 2020)

• Record Type: Journal Article
• Authors: Tang, Mou; Wang, Yanan; Zhou, Wenting; Yang, Mian; Liu, Yi; Gong, Zhiwei
• Is Part Of: Bioresource technology. Volume 315(2020)
• Publication Date: 2020-11
• Subjects: Chitin -- Glucosamine -- N-acetylglucosamine -- Phosphate limitation -- Cutaneotrichosporon oleaginosum -- Microbial lipid; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2020.123897
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: GlcNAc, GlcN and acetate were compared for lipid production by C. oleaginosum. The theoretical lipid yields were calculated based on a small scale metabolic model. GlcN/acetate co-fermentation under phosphate limitation rendered higher lipid yield. GlcN and acetate were assimilated simultaneously. Lipid yield of 0.25 g/g was obtained from GlcNAc under phosphate limitation. Abstract: Chitin represents the second most abundant biomass after lignocelluloses in the biosphere. It can be depolymerized into either N -acetylglucosamine (GlcNAc) or glucosamine (GlcN) and acetate by different degradation strategies. However, these chitin-derived carbon sources have been scarcely compared for lipid production. Here, GlcNAc was found superior to GlcN or acetate for lipid accumulation by Cutaneotrichosporon oleaginosum . The lipid accumulation potential of these carbon sources was calculated based on a small scale metabolic model of C. oleaginosum . Co-fermentation of GlcN and acetate under phosphate limitation rendered improved lipid production. GlcN and acetate were assimilated simultaneously. The highest lipid titer and yield of 10.1 g/L and 0.25 g/g, respectively, was reached when GlcNAc was used under phosphate limitation. The fatty acids composition of the lipid samples showed similarities to vegetable oils, demonstrating the suitability in biodiesel industry. This study provides profitable guidance for the design of chitin-to-lipids routes.