Effects of antibacterial peptide-producing Bacillus subtilis and Lactobacillus buchneri on fermentation, aerobic stability, and microbial community of alfalfa silage. (November 2020)

• Record Type: Journal Article
• Authors: Bai, Jie; Xu, Dongmei; Xie, Dongmei; Wang, Musen; Li, Ziqian; Guo, Xusheng
• Is Part Of: Bioresource technology. Volume 315(2020)
• Publication Date: 2020-11
• Subjects: Bacillus subtilis -- Bacterial community -- Fungal community -- Aerobic exposure -- Alfalfa silage; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2020.123881
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Both L. buchneri (LB) and BS improved alfalfa silage quality and aerobic stability. LB and BS increased Lactobacillus but decreased Enterococcus in 60 d silage. LB and BS inhibited the growth of Enterococcus during aerobic exposure of silage. Candida and Pichia dominated the fungi in BS-treated silage after 9 d spoilage. LB treated silage had high fungi alpha diversity of after spoilage for 9 d. Abstract: This study assessed the effects of antibacterial peptide-producing Bacillus subtilis (BS), Lactobacillus buchneri (LB), or their combination on fermentation, proteolysis, aerobic stability, and microbial communities during ensiling and aerobic exposure phases of alfalfa silage. The results showed that the BS-treated silage displayed a higher lactic acid concentration, less proteolysis, and higher aerobic stability than those in the control silage. Both LB and BS treatments increased Lactobacillus and Ascochyta abundance, and decreased Enterococcus and Sporormiacea abundance after 60 d of fermentation. LB and BS also inhibited the growth of Enterococcus after 3 d of aerobic exposure but similar to the control silage, the fungal community of BS silage was dominated by Candida and Pichia after 9 d of aerobic exposure. Therefore, inoculation of BS improved silage fermentation quality, aerobic stability and bacterial community during ensiling and after 3 d of aerobic exposure.