TLR13, TLR22, TRAF6, and TAK1 in the soiny mullet (Liza haematocheila): Molecular characterization and expression profiling analysis. (November 2020)

• Record Type: Journal Article
• Authors: Qi, Zhitao; Xu, Yang; Wang, Xin; Wang, Sisi; Zhang, Qihuan; Wang, Zisheng; Gao, Qian
• Is Part Of: Developmental and comparative immunology. Volume 112(2020)
• Publication Date: 2020-11
• Subjects: Liza haematocheila -- Toll-like receptor -- TRAF6 -- TAK1 -- Expression -- Bacterial infection; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2020.103774
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: Toll-like receptors (TLRs) and their associated signaling pathways play pivotal roles in the immune response to invading pathogens. Here, TLR13, TLR22, tumor necrosis factor receptor-associated factor 6 (TRAF6), and transforming growth factor-β-activated kinase1 (TAK1) were characterized in the soiny mullet ( Liza haematocheila ), representative mugilid species that is widely cultured in Asia. The four mullet genes, which shared characteristic features with their counterparts in other teleosts, were ubiquitously expressed in all of the examined tissues, albeit with different expression patterns. Following Streptococcus dysgalactiae infection, the four genes were upregulated to different degrees in various mullet tissues. These results indicated that the four genes were involved in the mullet immune response to bacterial infection. To the best of our knowledge, this is the first characterization of these four genes in mullet. Our results provide a basis for future studies of TLR signaling pathways in mullet, as well as for similar studies in other mugilids. Highlights: Two TLRs (TLR13 and TLR22) and two TLR signal transducers (TRAF6 and TAK1) are obtained from soiny mullet. This paper is the first report on these four genes of Mugiliformes. This paper investigates the expression of these four genes under gram positive bacterial infection.