Microbial community shift and functional genes in response to nitrogen loading variations in an anammox biofilm reactor. (September 2020)

• Record Type: Journal Article
• Authors: Liu, Lingjie; Ji, Min; Wang, Fen; Yan, Zhao; Tian, Zhongke; Wang, Siyu; Wang, Shuya
• Is Part Of: International biodeterioration & biodegradation. Volume 153(2020)
• Publication Date: 2020-09
• Subjects: Anammox -- Nitrogen loading rate -- Microbial community -- Functional genes; Biodegradation -- Periodicals; Bioremediation -- Periodicals; Biodegradation -- Periodicals; Biodégradation -- Périodiques; Biorestauration -- Périodiques; Electronic journals; 620.11223
• DOI: 10.1016/j.ibiod.2020.105023
• Languages: English
• ISSNs: 0964-8305

Abstract

Abstract: A lab-scale anaerobic ammonium oxidation (anammox) biofilm reactor was operated to explore the impact of free ammonia (FA) and free nitrous acid (FNA) on the anammox process with nitrogen loading rate (NLR) increasing from 0.1 to 0.5 g/L.d with identical pH of 7.8. When influent NLR was less than 0.4 g/L.d, total inorganic nitrogen (TIN) removal efficiency maintained around 80% on average. When the NLR increased to 0.5 g/L.d, TIN removal efficiency sharply reduced to 66%. It was revealed that Candidatus Brocadia and Candidatus Kuenenia were the main anammox genera in this study. When NLR raised from 0.1 to 0.5 g/L.d, together with FA from 4.44 to 20.23 mg/L and FNA from 5.00 to 22.69 μg/L, the dominant anammox genus showed a shift from Candidatus Kuenenia to Candidatus Brocadia. The highest anammox community abundance of 25.3% and maximum absolute abundances of anammox-related functional genes were observed at NLR of 0.3 g/L.d, together with 12.88 mg/L FA and 14.58 μg/L FNA. Graphical abstract: Image 1 Highlights: Nitrogen removal efficiency maintained at 80% within NLR of 0.4 g/L.d and reduced to 66% at NLR of 0.5 g/L.d. The dominant anammox genus shifted from Candidatus Kuenenia to Candidatus Brocadia with NLR, FA and FNA increasing. Anammox-related functional genes and communities showed the highest abundance at NLR of 0.3 g/L.d.