Polyethyleneimine as a tool for compounds fractionation by flocculation in a microalgae biorefinery context. (November 2020)

• Record Type: Journal Article
• Authors: Ba, Fatou; Foissard, Alexandrine; Lebert, André; Djelveh, Gholamreza; Laroche, Céline
• Is Part Of: Bioresource technology. Volume 315(2020)
• Publication Date: 2020-11
• Subjects: Microalgae -- Biorefinery -- Polyethyleneimine -- Flocculation; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2020.123857
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Polyethyleneimine can be used for fractionation of H. pluvialis compounds. Water-soluble and fat-soluble fractions can easily and efficiently be separated. 95% of fat-soluble compounds can be recovered in the pellet. 100% of sugars and 90% of proteins remained in the supernatant. Technofunctional properties of the water-soluble fraction were not altered. Abstract: In the context of emerging biorefinery for microalgae, polyethyleneimine (PEI), has been tested in order to achieve separation of fat-soluble and water-soluble compounds from Haematococcus pluvialis . Several parameters were taken into account (ratio between sample and PEI, pH, and ionic strength) and 2 conditions (0.075% PEI pH 7.4, and 0.100% PEI pH8.5) were studied for up-scalability, with a recovery of flocculated compounds (lipids and pigments), and a complete characterization of both phases. Using 0.075% PEI, pH7.4, 100% sugars and 89.8% proteins were retained in the supernatant, but some trace of beta-carotene were also detected. For 0.100% PEI, pH 8.5, a loss in proteins content was highlighted (61.2% proteins retained), but no residual lipids or pigments were detected. PEI could therefore be considered as an efficient method to fractionate fat-soluble and water-soluble compounds from microalgae.