Process optimization of fucoxanthin production with Tisochrysis lutea. (November 2020)

• Record Type: Journal Article
• Authors: Gao, Fengzheng; Teles (Cabanelas, ITD), Iago; Wijffels, René H; Barbosa, Maria J
• Is Part Of: Bioresource technology. Volume 315(2020)
• Publication Date: 2020-11
• Subjects: Fucoxanthin -- Tisochrysis lutea -- Temperature -- Dilution rate -- Absorbed light; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2020.123894
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Process optimization of fucoxanthin production. Highlights: Highest ever reported fucoxanthin (Fx) productivity in Tisochrysis (9.81 mg L −1 d −1 ). Optimum light at 300 μmol m −2 s −1 and temperature at 30 °C for Fx productivity. Fx content (3.68–16.39 mg/g) increases with decreasing daily light absorbed. High dilution rate (0.53, 0.80 d −1 ) leads to higher Fx productivity. Abstract: To optimize fucoxanthin production in Tisochrysis lutea, the effect of different process parameters on fucoxanthin productivity (Pfx) were evaluated using batch and continuous experiments. In batch, the highest Pfx was found at 30 °C and 300 μmol m −2 s −1, allowing to design continuous experiments to optimize the dilution rate. The highest ever reported Pfx (9.43–9.81 mg L −1 d −1 ) was achieved at dilution rates of 0.53 and 0.80 d −1 . Irradiance was varied (50–500 μmol m −2 s −1 ) to result in a range of absorbed light between 2.23 and 25.80 mol m −2 d −1 at a fixed dilution rate (0.53 d −1 ). These experiments validated the hypothesis that light absorbed can be used to predict fucoxanthin content, resulting in 2.23 mol m −2 d −1 triggering the highest fucoxanthin content (16.39 mg/g). The highest Pfx was found with 18.38 mol m −2 d −1 . These results can be used to achieve high Pfx or fucoxanthin content during cultivation of Tisochrysis lutea .