DNA synthesis increases during the first hours post-emergence in Anopheles albimanus mosquito midgut. (November 2020)

• Record Type: Journal Article
• Authors: Maya-Maldonado, Krystal; Cardoso-Jaime, Victor; Hernández-Martínez, Salvador; Vázquez-Calzada, Carlos; Hernández-Hernández, Fidel de la Cruz; Lanz-Mendoza, Humberto
• Is Part Of: Developmental and comparative immunology. Volume 112(2020)
• Publication Date: 2020-11
• Subjects: Cell cycle -- Mosquitoes -- Midgut; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2020.103753
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: In hematophagous insects, the midgut is a fundamental barrier against infections and limits the development and transmission of pathogens. However, in mosquitoes, cell differentiation, proliferation, and cell cycle process in the midgut have not been characterized. Here we provide evidence of how cell cycle progression occurs in the newly emerged Anopheles albimanus mosquito midgut and describing cyclins expression as mediators of the cell cycle. The cell cycle at different post-emergence times was evaluated in disaggregated cells from midgut tissue using flow cytometry. Also, cyclins A, B, and E were identified by bioinformatics tools. These cyclins were used to analyze cell cycle progression. Flow cytometry data and the expression-pattern of the cyclins by qRT-PCR supported a polyploidy process, besides mitosis marker was marginally detected and only in newly emerged mosquitoes. Our results suggest that DNA increment in midguts occurs by polyploidy during the first hours post-emergence. Highlights: An. albimanus cyclins sequences are conserved in mosquitoes and other insect species. In An. albimanus midgut DNA content increases by polyploidy. Twelve hours after emergence are crucial to increase DNA content. Midgut cell DNA content analysis was done by flow cytometry using disaggregate cells.