Effect of mesophilic anaerobic digestion on the resistome profile of dairy manure. (November 2020)

• Record Type: Journal Article
• Authors: Flores-Orozco, Daniel; Patidar, Rakesh; Levin, David B.; Sparling, Richard; Kumar, Ayush; Çiçek, Nazim
• Is Part Of: Bioresource technology. Volume 315(2020)
• Publication Date: 2020-11
• Subjects: Anaerobic digestion -- Dairy manure -- Antibiotic resistance genes -- Resistome -- Metagenomics; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2020.123889
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: 187 unique antibiotic-resistance genes (ARGs) were detected in dairy manures. The relative abundance of several ARGs was reduced during anaerobic digestion. Enrichment of ARGs for glycopeptides and nucleosides was observed. Changes in ARG levels were driven by shifts in the microbial community. Bioinformatic analyses revealed the presence of potential microbial ARG hosts. Abstract: The effect of mesophilic anaerobic digestion (AD) on the resistome profile of manures from two different dairy farms was evaluated using a metagenomic approach. A total of 187 unique Antibiotic resistance genes (ARGs) for 17 different classes of antibiotics were detected in raw (undigested) manures. The results indicate that regardless of the origin of the dairy manure, mesophilic AD was capable of reducing or enriching the relative abundance of some ARGs. The main driver of these changes was strongly correlated with the evolution of the microbial community during the AD process. Putative ARG hosts were suggested by analyses of the co-occurrence of microbial groups and ARGs. Finally, network analyses revealed that mesophilic AD could also reduce the co-occurrence of different groups of ARGs potentially located in the same genetic elements. Our results provide valuable insights into the microbial mechanisms driving the diversity and abundance of ARGs during mesophilic AD.