Anion exchange chromatography of oligonucleotides under denaturing conditions. (2nd June 2020)

• Record Type: Journal Article
• Authors: Sun, Jinyu; Joshi, Dhruval; Betancourt, Frank; Solodinin, Andrei; Woodland, Breyer; Yan, Hongbin
• Is Part Of: Nucleosides, nucleotides & nucleic acids. Volume 39:Number 6(2020)
• Page Start: 818
• Page End: 828
• Publication Date: 2020-06-02
• Subjects: Anion exchange HPLC -- denaturing -- secondary structure -- self-complementary -- polymerase chain reaction; Nucleosides -- Periodicals; Nucleotides -- Periodicals; Nucleic acids -- Periodicals; 572.8
• DOI: 10.1080/15257770.2019.1706096
• Languages: English
• ISSNs: 1525-7770

Abstract

Abstract: Denaturing anion exchange HPLC simplifies the chromatographic profiles of self-complementary sequences such as d(CG)6, d(CG)6 with locked nucleic acid modifications, d(AT)15, and polymerase chain reaction mixtures. These chromatographic conditions use eluents containing up to 4 M urea at pH 12.4, and lead to the abolishment of secondary structures and meaningful chromatographic patterns of self-complementary sequences. Similarly, PCR template, FAM-labelled primer and FAM-labelled PCR products were resolved, making interpretation of PCR reaction products possible.