Preparation, identification, and functional analysis of monoclonal antibodies against atypical porcine pestivirus NS3 protein. (September 2020)

• Record Type: Journal Article
• Authors: Yan, Miaomiao; Huang, Jingling; Chen, Jianing; Yang, Wenting; Liu, Guangliang
• Is Part Of: Journal of veterinary diagnostic investigation. Volume 32:Number 5(2020:Sep.)
• Page Start: 695
• Page End: 699
• Publication Date: 2020-09
• Subjects: atypical porcine pestivirus -- monoclonal antibodies -- NS3 protein; Veterinary medicine -- Diagnosis -- Periodicals; 636.0896075
• DOI: 10.1177/1040638720939923
• Languages: English
• ISSNs: 1040-6387

Abstract

Atypical porcine pestivirus (APPV) had been detected in many countries. However, to date, a commercial detection kit is not available because of a lack of specific monoclonal antibodies (mAbs) to APPV. We generated 7 mAbs targeting the NS3 protein of APPV. Isotyping results indicated that all of these mAbs are IgG1 with a kappa light chain. We analyzed the epitopes recognized by mAbs 2B6, 6G11, 8D1, 8D3, and 8F12, which recognized the same linear epitope (GRIKSAYSDE); the 6H3 and 7E10 mAbs recognized 2 different conformational epitopes. Applications of these antibodies were verified by ELISA, western blot, indirect immunofluorescence assay, and flow cytometry. The antibodies were functionally workable for these immunoassays except for 8F12, which could not be used in flow cytometry.