In silico analysis for development of epitopes-based peptide vaccine against Alkhurma hemorrhagic fever virus. Issue 10 (2nd July 2020)

• Record Type: Journal Article
• Authors: Ul-Rahman, Aziz; Shabbir, Muhammad Abu Bakr
• Is Part Of: Journal of biomolecular structure & dynamics. Volume 38:Issue 10(2020)
• Page Start: 3110
• Page End: 3122
• Publication Date: 2020-07-02
• Subjects: Alkhurma hemorrhagic fever virus -- immunoinformatics approaches -- envelope protein -- B-cells and T-cells epitopes -- peptide vaccine -- humoral and cell-mediated immunity; Biomolecules -- Periodicals; Molecular structure -- Periodicals; Molecular Biology -- Periodicals; Biomechanics -- Periodicals; 572
• DOI: 10.1080/07391102.2019.1651673
• Languages: English
• ISSNs: 0739-1102

Abstract

Abstract: Alkhurma hemorrhagic fever virus (ALKV) causes a fatal clinical disease in human beings of different tropical and sub-tropical regions. Recently, the ALKV epidemics have raised a great public health concern with the room for improvement in the essential therapeutic interventions. Despite increased realistic clinical cases of ALKV infection, the efficient vaccine or immunotherapy is not yet available to-date. Therefore, the current study aimed to analyze the envelope glycoprotein of ALKV for the development of B-cells and T-cells epitope-based peptide vaccine using the computational in silico method. Utilizing various immunoinformatics approaches, a total of 5 B-cells and 25 T-cells (MHC-I = 17, MHC-II = 8) epitope-based peptides were predicted in the current study. All predicted peptides had highest antigenicity and immunogenicity scores along with high binding affinity to human leukocyte antigen (HLA) class II alleles. Among 25T-cell epitopes, three peptides were found alike to have affinity to bind both MHC-I and MHC-II alleles. These outcomes suggested that these predicted epitopes could potentially be used in the development of an efficient vaccine against ALKV, which may enable to elicit both humoral and cell-mediated immunity. Although, these predicted peptides could be useful in designing a candidate vaccine for the prevention of ALKV; however, it's in vitro and in vivo assessments are prerequisite. Communicated by Ramaswamy H. Sarma