The effect of the macrophage migration inhibitory factor (MIF) on excisional wound healing in vivo. Issue 3 (1st June 2020)

• Record Type: Journal Article
• Authors: Kim, Bong-Sung; Breuer, Benjamin; Arnke, Kevin; Ruhl, Tim; Hofer, Tanja; Simons, David; Knobe, Matthias; Ganse, Bergita; Guidi, Marco; Beier, Justus P.; Fuchs, Paul C.; Pallua, Norbert; Bernhagen, Jürgen; Grieb, Gerrit
• Is Part Of: Journal of plastic surgery and hand surgery. Volume 54:Issue 3(2020)
• Page Start: 137
• Page End: 144
• Publication Date: 2020-06-01
• Subjects: MIF -- wound healing -- flap model -- excisional wound -- perfusion -- hypoxia -- macrophages; Surgery -- Periodicals; Hand -- Surgery -- Periodicals; Orthopedics -- Periodicals; Surgery, Plastic -- Periodicals; 617.95
• DOI: 10.1080/2000656X.2019.1710710
• Languages: English
• ISSNs: 2000-656X

Abstract

Abstract: Background: The macrophage migration inhibitory factor (MIF) has been determined as a cytokine exerting a multitude of effects in inflammation and angiogenesis. Earlier studies have indicated that MIF may also be involved in wound healing and flap surgery. Methods: We investigated the effect of MIF in an excisional wound model in wildtype, Mif -/- and recombinant MIF treated mice. Wound closure rates as well as the macrophage marker Mac-3, the pro-inflammatory cytokine tumor necrosis factor α (TNFα) and the pro-angiogenic factor von Willebrand factor (vWF) were measured. Finally, we used a flap model in Mif -/- and WT mice with an established perfusion gradient to identify MIF's contribution in flap perfusion. Results: In the excision wound model, we found reduced wound healing after MIF injection, whereas Mif deletion improved wound healing. Furthermore, a reduced expression of Mac-3, TNF α and vWF in Mif -/- mice was seen when compared to WT mice. In the flap model, Mif -/- knockout mice showed mitigated flap perfusion with lower hemoglobin content and oxygen saturation as measured by O2 C measurements when compared to WT mice. Conclusions: Our data suggest an inhibiting effect of MIF in wound healing with increased inflammation and perfusion. In flaps, by contrast, MIF may contribute to flap vascularization.