Development and applicability of GBS approach for genomic studies in Japanese plum (Prunus salicina Lindl.). Issue 3 (4th May 2019)

• Record Type: Journal Article
• Authors: Salazar, Juan Alfonso; Pacheco, Igor; Silva, Claudia; Zapata, Patricio; Shinya, Paulina; Ruiz, David; Martínez-Gómez, Pedro; Infante, Rodrigo
• Is Part Of: Journal of horticultural science & biotechnology. Volume 94:Issue 3(2019)
• Page Start: 284
• Page End: 294
• Publication Date: 2019-05-04
• Subjects: Japanese plum -- breeding -- genetic diversity -- phenology -- fruit quality -- Prunus salicina -- SNP -- sequencing; Horticulture -- Periodicals; Plant biotechnology -- Periodicals; Biotechnology -- Periodicals; Biotechnology; Horticulture; Plant biotechnology; Periodicals; 635.05
• DOI: 10.1080/14620316.2018.1543559
• Languages: English
• ISSNs: 1462-0316

Abstract

ABSTRACT: Genotyping by sequencing (GBS) provides a large quantity of useful data suitable for the identification of single nucleotide polymorphisms (SNPs), facilitating accurate genomic studies in plant species. In this study, GBS-based SNPs were used to characterise 11 Japanese plum cultivars and to explore their natural allelic diversity in relation to the most important phenology events (flowering date, ripening date and fruit development period) and fruit quality traits (weight, shape, skin and flesh colour, over colour, skin and flesh chlorophyll index, flesh firmness and soluble solids concentration). GBS-based SNPs were shown to be a powerful tool for genetic diversity and other genomic studies where SNP markers were related to several traits, particularly for flowering date, ripening date, fruit development period, skin chlorophyll degradation, flesh chlorophyll degradation and flesh colour. These results represent a preliminary approach using GBS as a possible breeding tool in current and new Japanese plum breeding programmes.