Development of a multiparent advanced generation intercross (MAGIC) population for genetic exploitation of complex traits in Brassica juncea: Glucosinolate content as an example. (6th April 2020)

• Record Type: Journal Article
• Authors: Yan, Wei; Zhao, Haifei; Yu, Kunjiang; Wang, Tianya; Khattak, Aimal Nawaz; Tian, Entang
• Editors: Snowdon, Rod
• Is Part Of: Plant breeding. Volume 139:Number 4(2020)
• Page Start: 779
• Page End: 789
• Publication Date: 2020-04-06
• Subjects: Brassica juncea -- MAGIC population -- plant breeding -- population structure -- QTL mapping; Plant breeding -- Periodicals; 631.52
• DOI: 10.1111/pbr.12820
• Languages: English
• ISSNs: 0179-9541

Abstract

Abstract: Multiparent advanced generation intercross (MAGIC) populations have recently been developed to allow the high‐resolution mapping of complex quantitative traits. This article describes the development of one MAGIC population and verifies its potential application for mapping quantitative trait loci (QTLs) in Brassica juncea . A total of 170 RILs were genotyped using 346 intron length polymorphism (ILP) polymorphic markers. The population lacks significant signatures of population structure that are suitable for the analysis of complex traits. Genome‐wide association mapping (GWAS) identified three major glucosinolate (GSL) QTLs of QGsl.ig01.1 on J01 for indole GSL (IG), QGsl.atg09.1 on J09 and QGsl.atg11.1 on J11 for aliphatic GSL (AG) and total GSL (TG). The candidate genes for QGsl.ig01.1, QGsl.atg09.1 and QGsl.atg11.1 are GSH1, GSL‐ALK and MYB28, which are involved in converting glutamate and cysteine to γ ‐EC, the accumulation of glucoraphanin and the whole process of AG metabolism, respectively. In addition to its QTL mapping purpose, the MAGIC population could also be potentially utilized in variety development by breeders.