Identification of the minimum region of Bordetella pertussis Vag8 required for interaction with C1 inhibitor. (2nd July 2020)

• Record Type: Journal Article
• Authors: Onoda, Naoki; Hiramatsu, Yukihiro; Teruya, Shihono; Suzuki, Koichiro; Horiguchi, Yasuhiko
• Is Part Of: Microbiology and immunology. Volume 64:Number 8(2020)
• Page Start: 570
• Page End: 573
• Publication Date: 2020-07-02
• Subjects: Bordetella pertussis -- C1 inhibitor -- Vag8; Microbiology -- Periodicals; Immunology -- Periodicals; Allergy and Immunology -- Periodicals; Microbiology -- Periodicals; Microbiologie -- Périodiques; Immunologie -- Périodiques; 579
• DOI: 10.1111/1348-0421.12799
• Languages: English
• ISSNs: 0385-5600

Abstract

Abstract: An autotransporter of Bordetella pertussis, virulence‐associated gene 8 (Vag8), binds and inactivates the complement regulator, C1 inhibitor (C1‐Inh), and plays a role in evasion of the complement system. However, the molecular interaction between Vag8 and C1‐Inh remains unclear. Here, we localized the minimum region of Vag8 required for interaction with C1‐Inh by examining the differently truncated Vag8 derivatives for the ability to bind and inactivate C1‐Inh. The truncated Vag8 containing amino‐acid residues 102–548, but not 102–479 and 202–648, showed the full activity of intact Vag8, suggesting that the separate 102–202 and 548–648 amino‐acid regions of Vag8 mediate the interaction with C1‐Inh.