CaMKII inhibitor 1 (CaMK2N1) mRNA is upregulated following LTP induction in hippocampal slices. Issue 10 (31st May 2020)

• Record Type: Journal Article
• Authors: Astudillo, Daniela; Karmelic, Daniel; Casas, Barbara S.; Otmakhov, Nikolai; Palma, Veronica; Sanhueza, Magdalena
• Is Part Of: Synapse. Volume 74:Issue 10(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-05-31
• Subjects: CaMK2N1 -- CaMK2N2 -- CaMKII -- gene expression -- LTP; Synapses -- Periodicals; 612
• DOI: 10.1002/syn.22158
• Languages: English
• ISSNs: 0887-4476

Abstract

Abstract: CaMK2N1 and CaMK2N2 (also known as CaMKIINα and β) are endogenous inhibitors of calcium/calmodulin‐dependent kinase II (CaMKII), an enzyme critical for memory and long‐term potentiation (LTP), a form of synaptic plasticity thought to underlie learning. CaMK2N1/2 mRNAs are rapidly and differentially upregulated in the hippocampus and amygdala after acquisition or retrieval of fear memory. Moreover, CaMK2N2 protein levels increase after contextual fear conditioning. Therefore, it was proposed that CaMK2N1/2 genes ( Camk2n1/2 ) could be immediate‐early genes transcribed promptly (30–60 min) after training. As a first approach to explore a role in synaptic plasticity, we assessed a possible regulation of Camk2n1/2 during the expression phase of LTP in hippocampal CA3–CA1 connections in rat brain slices. Quantitative PCR revealed that Camk2n1, but not Camk2n2, is upregulated 60 min after LTP induction by Schaffer collaterals high‐frequency stimulation. We observed a graded, significant positive correlation between the magnitude of LTP and Camk2n1 change in individual slices, suggesting a coordinated regulation of these properties. If mRNA increment actually resulted in the protein upregulation in plasticity‐relevant subcellular locations, CaMK2N1 may be involved in CaMKII fine‐tuning during LTP maintenance or in the regulation of subsequent plasticity events (metaplasticity). Abstract : CaMKII inhibitor 1 (CaMK2N1) mRNA is upregulated 1 hr after inducing LTP in acute rat hippocampal slices. CaMK2N1 mRNA increase is correlated with the magnitude of LTP.