Effects of Medium pH and Preconditioning Treatment on Protein Adsorption on 45S5 Bioactive Glass Surfaces. Issue 15 (18th May 2020)

• Record Type: Journal Article
• Authors: Höhn, Sarah; Zheng, Kai; Romeis, Stefan; Brehl, Martin; Peukert, Wolfgang; de Ligny, Dominique; Virtanen, Sannakaisa; Boccaccini, Aldo R.
• Is Part Of: Advanced materials interfaces. Volume 7:Issue 15(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-05-18
• Subjects: 45S5 Bioglass -- bovine serum albumin -- pH influence -- preconditioning -- surface reactivity; Materials science -- Periodicals; 620.11
• DOI: 10.1002/admi.202000420
• Languages: English
• ISSNs: 2196-7350

Abstract

Abstract: Protein adsorption on 45S5 bioactive glass (BG, Bioglass) surfaces influences the biocompatibility of Bioglass and the cellular response to the material. The medium pH greatly affects protein adsorption behavior. However, the influence of pH variation on protein adsorption on Bioglass has not been investigated in detail before, although an acidifying pH has been observed in fractured or injured bone tissues. This study investigates how the medium pH (pH 7, 5, and 2) affects protein (serum albumin) adsorption on Bioglass with or without preconditioning in simulated body fluid (SBF). The results show that Bioglass can adsorb a larger amount of bovine serum albumin (BSA) than bioinert glasses at all tested pHs. The BSA adsorption on Bioglass surfaces is pH‐dependent and a larger amount of adsorbed BSA is observed at lower pH (5 and 2). After preconditioning, BSA adsorption is significantly enhanced. However, the trend of pH‐dependent adsorption is attenuated. No significant difference in BSA adsorption is observed at different pHs after preconditioning. The results reveal for the first time the influence of medium pH on protein adsorption on Bioglass with or without preconditioning treatment in SBF, which provides useful information for developing Bioglass based biomedical devices that will be in contact with protein‐containing physiological fluids during applications. Abstract : Protein adsorption determines biocompatibility and cellular attachment on 45S5 bioactive glass (BG) surfaces. The influence of medium pH on albumin adsorption on bioactive and inert glasses is investigated. At lower pHs, BG adsorbs a larger amount of albumin. Preconditioning BGs in simulated body fluid reduces the pH‐dependent protein adsorption due to the formation of hydroxyapatite crystals on BG surfaces.