Effects of anti-wrinkle and skin-whitening fermented black ginseng on human subjects and underlying mechanism of action. Issue 11 (17th June 2020)

• Record Type: Journal Article
• Authors: Park, Jin Ju; An, Junmin; Lee, Jung Dae; Kim, Hyang Yeon; Im, Jueng Eun; Lee, Eunyoung; Ha, Jaehyoun; Cho, Chang Hui; Seo, Dong-Wan; Kim, Kyu-Bong
• Is Part Of: Journal of toxicology and environmental health. Volume 83:Issue 11/12(2020)
• Page Start: 470
• Page End: 484
• Publication Date: 2020-06-17
• Subjects: Fermented black ginseng -- cosmetics -- anti-wrinkle -- whitening -- MMP-1; Toxicology -- Periodicals; Environmental health -- Periodicals; 615.90205
• DOI: 10.1080/15287394.2020.1777492
• Languages: English
• ISSNs: 1528-7394

Abstract

ABSTRACT: The aim of this study was to determine the effects of anti-wrinkle and skin-whitening of fermented black ginseng (FBG) in human subjects and to examine underlying biochemical mechanisms of action. A clinical study was performed to evaluate efficacy and safety using a 1% FBG cream formulation. Twenty-three subjects were recruited and instructed to apply control or FBG creams each on half of their face twice daily for 8 weeks. After 8 weeks, FBG cream significantly reduced the appearance of eye wrinkles compared to prior to exposure and control cream. Skin color was significantly brightened using FBG cream in comparison with a control cream. To determine the mechanism of actions involved in anti-wrinkle and skin-whitening effects various concentrations of FBG were applied to human fibroblast CCD-986sk and mouse melanoma B16F1 cells. Collagen synthesis in CCD-986sk cells was improved significantly at 1, 3, 10, or 30 µg/ml of FBG. At 30 µg/ml, FBG significantly inhibited (73%) collagenase, and matrix metalloproteinase-1 (MMP-1) compared to control. Tyrosinase activity and DOPA (3, 4-dihydroxy-L-phenylalanine) oxidation were significantly decreased at all tested concentrations. Melanin production in B16F1 cells was concentration-dependently reduced from 15% to 60% by all concentrations of FBG. These results suggested that a 1% FBG cream exerted anti-wrinkle and skin-whitening effects.