Selective cross-linking behavior of oligodeoxyribonucleotides containing 2'-O-[N-(4, 5', 8-trimethylpsoralen-4'-ylmethylcarbamoyl)]adenosine to mutant H-ras DNA. (20th February 2020)

• Record Type: Journal Article
• Authors: Yamayoshi, Asako; Higuchi, Maiko; Sakai, Yui; Kobori, Akio; Yamamoto, Tsuyoshi; Shibata, Takayuki; Murakami, Akira
• Is Part Of: Nucleosides, nucleotides & nucleic acids. Volume 39:Number 1-3(2020)
• Page Start: 119
• Page End: 130
• Publication Date: 2020-02-20
• Subjects: Photo-responsive oligonucleotide -- psoralen -- point mutation -- H-ras; Nucleosides -- Periodicals; Nucleotides -- Periodicals; Nucleic acids -- Periodicals; 572.8
• DOI: 10.1080/15257770.2019.1677912
• Languages: English
• ISSNs: 1525-7770

Abstract

Abstract: Point mutations are well characterized activators of oncogenes but are often indistinguishable using common gene technologies. In general, the precise sites of point-mutated oncogenes are difficult to distinguish under physiological conditions primarily because single nucleotide mismatch do not affect the annealing temperatures of DNA probes sufficiently. To address this limitation, we developed photo-responsive oligodeoxyribonucleotides containing 2′- O -[ N -(4, 5′, 8-trimethylpsoralen-4'-ylmethylcarbamoyl)]adenosine (Ps-amd-Oligo), which can be used to selectively manipulate and identify genes with point mutations. Here we present time course analyses of the photo-cross-linking efficiency of Ps-amd-Oligo with DNA and RNA and show promising selectivity for the oncogene H-ras .