Gram-negative Microbiota Blooms in Premature Twins Discordant for Parenteral Nutrition-associated Cholestasis. Issue 5 (May 2020)

• Record Type: Journal Article
• Authors: Hourigan, Suchitra K.; Moutinho, Thomas J.; Berenz, Andrew; Papin, Jason; Guha, Pallabi; Bangiolo, Lois; Oliphant, Sandra; Provenzano, Marina; Baveja, Raj; Baker, Robin; Vilboux, Thierry; Levy, Shira; Deopujari, Varsha; Nataro, James P.; Niederhuber, John E.; Moore, Sean R.
• Is Part Of: Journal of pediatric gastroenterology and nutrition. Volume 70:Issue 5(2020)
• Publication Date: 2020-05
• Subjects: cholestasis -- microbiota -- neonate -- parenteral nutrition; Children -- Nutrition -- Periodicals; Pediatric gastroenterology -- Periodicals; Infants -- Nutrition -- Periodicals; Nutrition disorders in children -- Periodicals; Child Nutrition -- Periodicals; Digestive System -- growth & development -- Periodicals; Gastrointestinal Diseases -- Periodicals; Infant Nutrition -- Periodicals; Nutrition Disorders -- Periodicals; Child; 618.923
• DOI: 10.1097/MPG.0000000000002617
• Languages: English
• ISSNs: 0277-2116

Abstract

Abstract : ABSTRACT: Parenteral nutrition-associated cholestasis (PNAC) causes serious morbidity in the neonatal intensive care unit. Infection with gut-associated bacteria is associated with cholestasis, but the role of intestinal microbiota in PNAC is poorly understood. We examined the composition of stool microbiota from premature twins discordant for PNAC as a strategy to reduce confounding from variables associated with both microbiota and cholestasis. Eighty-four serial stool samples were included from 4 twin sets discordant for PNAC. Random Forests was utilized to determine genera most discriminatory in classifying samples from infants with and without PNAC. In infants with PNAC, we detected a significant increase in the relative abundance of Klebsiella, Veillonella, Enterobacter, and Enterococcus ( P < 0.05). Bray-Curtis dissimilarities in infants with PNAC were significantly different ( P < 0.05) from infants without PNAC. Our findings warrant further exploration in larger cohorts and experimental models of PNAC to determine if a microbiota signature predicts PNAC, as a basis for future interventions to mitigate liver injury. Abstract : Supplemental Digital Content is available in the text