Transcriptome profiling of two Dactylis glomerata L. cultivars with different tolerance in response to submergence stress. (July 2020)

• Record Type: Journal Article
• Authors: Zeng, Bing; Zhang, Yajie; Zhang, Ailing; Qiao, Dandan; Ren, Juncai; Li, Mingyang; Cai, Kai; Zhang, Jinhua; Huang, Linkai
• Is Part Of: Phytochemistry. Volume 175(2020)
• Publication Date: 2020-07
• Subjects: Dactylis glomerata L. -- Poaceae -- Submergence stress -- RNA sequencing -- Transcriptome -- Differently expressed gene; Botanical chemistry -- Periodicals; Biochemistry -- Periodicals; Botany -- Periodicals; Chimie végétale -- Périodiques; 572.2
• DOI: 10.1016/j.phytochem.2020.112378
• Languages: English
• ISSNs: 0031-9422

Abstract

Abstract: Submergence is one of the environmental stresses that limit plant growth and development. Dactylis glomerata L. is an important cool-season forage grass globally. To investigate the genes related to submergence response and the molecular mechanism associated with submergence tolerance, the transcriptome of D. glomerata in response to waterlogging treatment was analyzed. RNA-sequencing was performed in two D. glomerata cultivars, submergence tolerant 'Dianbei' and submergence sensitive 'Anba'. A total of 50, 045 unique genes matched the known proteins in the NCBI nr database by BLAST searches and 60.8% (30, 418) of these genes were annotated with GO terms. Among these, 1395 genes only differentially expressed in 'Dianbei' and 18 genes shown different expression all the time were detected between the submergence tolerant 'Dianbei' and sensitive 'Anba'. Gene ontology (GO) and KEGG pathway enrichment analyses demonstrated that the DEGs were mainly implicated in oxidation-reduction system, nucleic acid binding transcription factor activity, and glycerol kinase activity. The D. glomerata assembled transcriptome provided substantial molecular resource for further genomic analysis of forage grasses in response to submergence stress. The significant difference in expression of specific unigenes may account for waterlogging tolerance or acclimation in the two different D. glomerata cultivars. This study provided new insights into the molecular basis of submergence tolerance in D. glomerata . Graphical abstract: Image 1 Highlights: RNA-seq was performed in two Dactylis glomerata L. cultivars, submergence tolerant 'Dianbei' and sensitive 'Anba'. 1395 DEGs only expressed in 'Dianbei' and 18 genes showed different expression all the time between two cultivars. Enrichment analyses demonstrated that the DEGs were mainly implicated in oxidation-reduction system.