Transcriptome, bioaccumulation and toxicity analyses of earthworms (Eisenia fetida) affected by trifloxystrobin and trifloxystrobin acid. (October 2020)

• Record Type: Journal Article
• Authors: Liu, Tong; Liu, Yalei; Fang, Kuan; Zhang, Xiaolian; Wang, Xiuguo
• Is Part Of: Environmental pollution. Volume 265(2020)Part B
• Publication Date: 2020-10
• Subjects: Trifloxystrobin -- Trifloxystrobin acid -- Bioaccumulation -- Transcriptomic -- Biochemical toxicity; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2020.115100
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: As a promising fungicide, the potential environmental risk of trifloxystrobin (TFS) and its main metabolism trifloxystrobin acid (TFSA) in soil environment should be given special attention. The present study investigated the potential risks of TFS and TFSA in soil environment to earthworms ( Eisenia fetida ) through measuring several biomarkers. Residual analysis showed that TFSA was more stable than TFS in artificial soil with half-lives ranging from 138.6 to 231.0 d and 20.4–24.7 d, respectively. Additionally, the accumulation of TFS in earthworms increased in the beginning and then decreased from day 14, while that of TFSA continuously increased. At concentrations of 4.0 mg/kg and 10.0 mg/kg, the weight and lysosomal membrane stability of earthworms were reduced; however, the superoxide dismutase (SOD) activity, glutathione- S -transferase (GST) activity and malondialdehyde (MDA) content in earthworms were enhanced by TFS and TFSA. Moreover, the growth inhibition effect and the oxidative damage level induced by TFSA to earthworms were higher than those induced by TFS. The transcriptome analysis date indicated that the differentially expressed genes (DEGs) in both TFS and TFSA treatments were mainly enriched in ribosome pathway and lysosome pathway, finally affecting the protein synthesis and proteolysis in earthworms. The findings of the present study indicated that TFSA may pose a higher risk in the soil environment than TFS. Graphical abstract: Image 1 Highlights: Degradation and accumulation of TFS and TFSA in soil and earthworms were monitored. TFS and TFSA induced oxidative stress and differential gene expression in earthworms. TFS and TFSA had significant influences on ribosome and lysosome of earthworms. TFSA may have a higher risk to the soil environment than TFS.