Biosynthetic molecular imaging probe for tumor-targeted dual-modal fluorescence/magnetic resonance imaging. (October 2020)

• Record Type: Journal Article
• Authors: Zhao, Huaixin; Zhao, Huiting; Jiao, Yi; Zhu, Yi; Liu, Chunxia; Li, Feng; Wang, Yinsong; Gu, Zi; Yang, Dayong
• Is Part Of: Biomaterials. Volume 256(2020)
• Publication Date: 2020-10
• Subjects: Biosynthesis -- Fluorescence protein -- Dual-modal imaging -- Fluorescence imaging -- Magnetic resonance imaging; Biomedical materials -- Periodicals; Biocompatible Materials -- Periodicals; Biomatériaux -- Périodiques; 610.28
• DOI: 10.1016/j.biomaterials.2020.120220
• Languages: English
• ISSNs: 0142-9612

Abstract

Abstract: Molecular imaging enables noninvasive visualization of cancer-related biological processes for tumor diagnostics; engineering molecular probes for accurate and efficient tumor imaging remains challenge. Herein, by using a biosynthetic strategy, we prepared a tumor-targeted fluorescence/magnetic resonance protein-based probe for dual-modal imaging. The probe was biosynthesized by the fusion of targeting peptide (RGD) with fluorescence protein (RFP) and a small peptide (LBT) that had strong affinity with Gd 3+ . The probe showed excellent RGD-mediated tumor targeting and stable infrared emission, enabling sensitive tumor-specific imaging. Moreover, efficient T 1 -weighted magnetic resonance imaging was observed both in vitro and at tumor sites, facilitating high spatial resolution tumor imaging. The simultaneous dual-modal imaging provided more complementary information than each imaging modality. Our work demonstrates the capability of biosynthetic strategy to engineer molecular imaging probes for tumor-targeted multimodal bioimaging, and the probes can be customized to precisely localize and monitor other types of diseases. Graphical abstract: Image 1