Panton-Valentine leukocidin-positive methicillin-resistant Staphylococcus aureus correlates with disease severity in psoriasis: a case–control study. Issue 1 (2nd January 2019)

• Record Type: Journal Article
• Authors: Masallat, Doaa T.; Eldars, Waleed; State, Ahmed F.; Moemen, Dalia
• Is Part Of: Egyptian journal of basic and applied sciences. Volume 6:Issue 1(2019)
• Page Start: 149
• Page End: 157
• Publication Date: 2019-01-02
• Subjects: Psoriasis -- MRSA -- PSAI -- Panton-Valentinleukocidin; Science -- Periodicals; Technology -- Periodicals; Technology; Science; Science; Electronic journals; Periodicals; Periodical; 505
• DOI: 10.1080/2314808X.2019.1681662
• Languages: English
• ISSNs: 2314-808X

Abstract

ABSTRACT: Psoriasis is a chronic inflammatory cutaneous disorder, which is prone to methicillin-resistant Staphylococcus aureus (MRSA) infection. We aimed to to investigate prevalence of MRSA skin colonization among psoriatic patients and if there is a relation between Panton-Valentin leukocidin (PVL) expression and disease severity. A case-control study was conducted on 90 patients with active psoriatic lesions and 90 healthy controls. Scales from psoriasis plaques and skin swabs from healthy control were collected. Identification of MRSA and antibiotic susceptibility patterns were performed using routine microbiological methods. mecA and PVL genes were detected using PCR assay and pulsed field gel electrophoresis (PFGE) was done for MRSA typing. Prevalence of MRSA among psoriatic patients was 23.3%. Significant difference was noticed in MRSA colonization between psoriasis and control groups. Five (23.8%) MRSA isolates from skin lesions were positive for mecA and PVL genes. Antibiotic resistance among PVL negative MRSA was higher, specially towards clindamycin. Patients harboring PVL positive isolates had significantly lower mean age and higher PSAI score than those with negative isolates. Fourteen PVL negative isolated MRSA from psoriasis patients were the same pulsotype. There was significant MRSA colonization in psoriasis group and presence of PVL may contribute to increased severity of this disease.