OsDOG1L-3 regulates seed dormancy through the abscisic acid pathway in rice. (September 2020)

• Record Type: Journal Article
• Authors: Wang, Qian; Lin, Qibing; Wu, Tao; Duan, Erchao; Huang, Yunshuai; Yang, Chunyan; Mou, Changling; Lan, Jie; Zhou, Chunlei; Xie, Kun; Liu, Xi; Zhang, Xin; Guo, Xiuping; Wang, Jie; Jiang, Ling; Wan, Jianmin
• Is Part Of: Plant science. Volume 298(2020)
• Publication Date: 2020-09
• Subjects: ABA -- bZIP protein -- DOG1-like -- Oryza sativa -- Pre-harvest sprouting -- Seed dormancy; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2020.110570
• Languages: English
• ISSNs: 0168-9452

Abstract

Highlights: The N22 OsDOG1L-3 allele is a candidate gene for the seed dormancy in QTL qSd-1-1 . OsbZIP75 and OsbZIP78 promote OsDOG1L-3 expression through binding to its promoter. OsDOG1L-3 promotes the expression of ABA-related genes consistently with up-regulate ABA content. Abstract: Seed dormancy is closely related to pre-harvest sprouting resistance. Both plant hormone abscisic acid (ABA) and DELAY OF GERMINATION 1 (DOG1) protein are key regulators of seed dormancy. Their relationship is well reported in Arabidopsis, but little is known in rice. Here, we show that a quantitative trait locus, qSd-1-1 contributes significantly to seed dormancy differences between the strongly dormant indica variety N22 and non-dormant japonica variety Nanjing35. It encodes a DOG1-like protein named OsDOG1L-3 with homology to Arabidopsis DOG1. There were evident promoter and expression differences in OsDOG1L-3 between N22 and Nanjing35, and overexpression or introduction of the N22 OsDOG1L-3 allele in Nanjing35 enhanced its seed dormancy. OsDOG1L-3 expression was positively correlated with seed dormancy and induced by ABA. OsbZIP75 and OsbZIP78 bound directly with the promoter of OsDOG1L-3 to induce its expression. Overexpression of OsbZIP75 increased OsDOG1L-3 protein abundance and promoted seed dormancy. OsDOG1L-3 upregulated expression of ABA-related genes and increased ABA content. We propose that the N22 OsDOG1L-3 allele is a candidate gene for the seed dormancy in QTL qSd-1-1, and that it participates in the ABA pathway to establish seed dormancy in rice.