Distribution of Mpeg1+ cells in healthy grouper (Epinephelus coioides) and after Cryptocaryon irritans infection. Issue 104 (September 2020)

• Record Type: Journal Article
• Authors: Ni, Lu-Yun; Chen, Hong-Ping; Han, Rui; Luo, Xiao-Chun; Li, An-Xing; Li, Jia-Zhou; Dan, Xue-Ming; Li, Yan-Wei
• Is Part Of: Fish & shellfish immunology. Issue 104(2020)
• Page Start: 222
• Page End: 227
• Publication Date: 2020-09
• Subjects: Macrophage expressed gene 1 -- Macrophage -- Epinephelus coioides -- Cryptocaryon irritans; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2020.06.018
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Cryptocaryon irritans is an extremely harmful ciliated obligate parasite that is responsible for large economic losses in aquaculture. C. irritans infection can cause an insect-resistant immune response in fish, and many immune cells can be observed in the local infection site. However, it is unclear whether macrophages are involved in the host defense against C. irritans infection. The Mpeg1 protein can form pores and destroy the cell membrane of invading pathogens, and is also used as a macrophage-specific marker in mammals. Therefore, a polyclonal antibody against grouper recombinant Mpeg1a was produced to mark macrophages in this study, which could recognize both isoforms of Mpeg1 (Mpeg1a/b). Immunofluorescence revealed that EcMpeg1 positive cells were mostly distributed in the head kidney and spleen in healthy grouper. Immunofluorescence and immunohistochemistry showed that the number of EcMpeg1 positive cells increased in the gills after infection with C. irritans, implying that EcMpeg1 positive cells may be involved in the process of grouper resistance against C. irritans infection. Highlights: Rabbit anti-EcMpeg1a polyclonal antibody was produced to mark macrophages. Rabbit anti-EcMpeg1a antibody could recognize both isoforms of Mpeg1 (Mpeg1a/b). EcMpeg1-positive cells have phagocytic activity. EcMpeg1-positive cells were mostly distributed in the head kidney, and spleen in healthy grouper. The number of EcMpeg1-positive cells were increased in the gills after C. irritans infection.