Astaxanthin biosynthesis promotion with pH shock in the green microalga, Haematococcus lacustris. (October 2020)

• Record Type: Journal Article
• Authors: Han, Sang-Il; Chang, Sun Hyoung; Lee, Changsu; Jeon, Min Seo; Heo, Young Mok; Kim, Sok; Choi, Yoon-E
• Is Part Of: Bioresource technology. Volume 314(2020)
• Publication Date: 2020-10
• Subjects: Haematococcus lacustris -- Astaxanthin -- pH shock -- Biological change -- Mass culture; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2020.123725
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Haematococcus lacustris produces rich amounts of astaxanthin (AXT). AXT production was improved due to cellular physiology changes induced by pH shock. Gene expressions related to astaxanthin production were increased upon with pH shock. Other inductive techniques could be combined to increase AXT production. Abstract: In this study, the use of pH shock to improve astaxanthin synthesis in Haematococcus lacustris was investigated. It has been found that pH shock (pH = 4.5, 60 s) imposes stress in the cells and induces physiological changes, which result in astaxanthin accumulation. The optimal acid-base combination of pH shock was H2 SO4 -KOH, which increased the astaxanthin content per cell to 39 ± 6.92% than those of the control. In addition, pH shock can be applied simultaneously with the other inductive strategies such as high irradiance and carbon source supply. When high irradiance was applied simultaneously with pH shock, astaxanthin yield was increased 65 ± 0.541% than control. In addition, astaxanthin content per cell was increased 105 ± 6.66% than those of the control, with the concomitant application of carbon source addition with pH shock. Herein, these novel findings provide a useful technique for producing astaxanthin using H. lacustris .