Molecular mechanisms underlying lignocellulose degradation and antibiotic resistance genes removal revealed via metagenomics analysis during different agricultural wastes composting. (October 2020)

• Record Type: Journal Article
• Authors: Ma, Chaofan; Lo, Po Kim; Xu, Jiaqi; Li, Mingqi; Jiang, Zhiwei; Li, Gen; Zhu, Qiuhui; Li, Xintian; Leong, Siew Yoong; Li, Qunliang
• Is Part Of: Bioresource technology. Volume 314(2020)
• Publication Date: 2020-10
• Subjects: Composting -- Carbon sources -- Metagenomics -- Carbohydrate-active enzymes -- Lignocellulose degradation; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2020.123731
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: A better performance was shown in pig manure/bagasse pith composting. More abundant carbohydrate-active enzymes in bagasse pith compost were observed. A synergistical mechanism of lignocellulose degradation was revealed. Antibiotics and antibiotic resistance genes decreased significantly after composting. Abstract: In this study, the differences on the physico-chemical parameters, lignocellulose degradation, dynamic succession of microbial community, gene expression of carbohydrate-active enzymes and antibiotics resistance genes were compared during composting systems of bagasse pith/pig manure (BP) and manioc waste/pig manure (MW). The results revealed that biodegradation rates of organic matter, cellulose, hemicellulose and lignin (29.14%, 17.53%, 45.36% and 36.48%) in BP were higher than those (15.59%, 16.74%, 41.23% and 29.77%) in MW. In addition, the relative abundance of Bacillus, Luteimonas, Clostridium, Pseudomonas, Streptomyces and expression of genes encoding carbohydrate- active enzymes in BP were higher than those in MW based on metagenomics sequencing. During composting, antibiotics and antibiotic resistance genes were substantially reduced, but the removal efficiency was divergent in the both samples. Taken together, metagenomics analysis was a potential method for evaluating lignocellulose's biodegradation process and determining the elimination of antibiotic and antibiotic resistance genes from different composting sources of biomass.