Microbial diversity and fermentation profile of red clover silage inoculated with reconstituted indigenous and exogenous epiphytic microbiota. (October 2020)

• Record Type: Journal Article
• Authors: Ali, Niaz; Wang, Siran; Zhao, Jie; Dong, Zhihao; Li, Junfeng; Nazar, Mudasir; Shao, Tao
• Is Part Of: Bioresource technology. Volume 314(2020)
• Publication Date: 2020-10
• Subjects: Epiphytic microbiota -- Fermentation -- Microbial diversity -- Red clover -- Silage; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2020.123606
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Reconstitution mechanism of epiphytic microbiota was studied on red clover silage. Indigenous and exogenous epiphytic microbiota well-adapted on red clover silage. Pediococcus from red clover microbiota accelerate the lactic acid fermentation. Maize microbiota dominated by Lactobacillus in red clover terminal silage. Sorghum microbiota exhibited a broad microbial diversity in red clover silage. Abstract: The study investigated the effects of transplantation and reconstitution of indigenous and exogenous epiphytic microbiota on the fermentation quality and microbial community of red clover silage. Sterile red clover was inoculated with distilled water (RC0), extracted epiphytic microbiota of red clover (RC), maize (MZ), and sorghum (SG). RC inoculation rapidly decreased pH at the onset of ensiling. The LA concentration and ratio of LA/AA were higher in RC silage during entire ensiling while MZ silage during late phase of ensiling. Pediococcus was dominant in RC early silage, while Lactobacillus was abundant in MZ final silage. The SG terminal silage had higher pH (>4.50) and dominated by Sphingomonas, Enterobacter, and Novosphingobium. RC and MZ microbiota were beneficial in enhancing fermentation quality and microbial community in red clover silage. Transplantation and reconstitution of epiphytic microbiota can be a successful method to assess the effective and eco-friendly additive for the targeted crop.