TNF-α stimulates the expression of RANK during orthodontic tooth movement. (September 2020)

• Record Type: Journal Article
• Authors: Noguchi, Takahiro; Kitaura, Hideki; Ogawa, Saika; Qi, Jiawei; Shen, Wei-Ren; Ohori, Fumitoshi; Marahleh, Aseel; Nara, Yasuhiko; Pramusita, Adya; Mizoguchi, Itaru
• Is Part Of: Archives of oral biology. Volume 117(2020)
• Publication Date: 2020-09
• Subjects: OTM orthodontic tooth movement -- TNF tumor necrosis factor -- RANK receptor activator of nuclear factor-κB -- WT wild-type -- TNFRsKO TNFR1/TNFR2-deficient -- RANKL receptor activator of nuclear factor-κB ligand -- M-CSF macrophage colony-stimulating factor -- TRAP tartrate-resistant acid phosphatase; TNF-α -- RANK -- Orthodontic tooth movement -- Osteoclast -- Mouse; Mouth -- Periodicals; Mouth -- Diseases -- Periodicals; Dentistry -- Periodicals; Electronic journals; 617.6005
• DOI: 10.1016/j.archoralbio.2020.104796
• Languages: English
• ISSNs: 0003-9969

Abstract

Highlights: TNF-α enhanced RANK expression in osteoclast precursors in vitro . RANK expression is increased in TNF-α-injected mice. TNF-α induces RANK expression by orthodontic tooth movement. Abstract: Objective: This study aimed to evaluate the effects of tumor necrosis factor (TNF)-α on receptor activator of nuclear factor-κB (RANK) expression in osteoclast precursors in vitro and during orthodontic tooth movement (OTM) in vivo . Design: We assessed whether TNF-α influenced RANK expression levels in osteoclast precursors in vitro by real-time PCR and western blotting. For in vivo experiments, TNF-α was subcutaneously injected into mouse calvariae daily for 5 days. Mice were sacrificed and RANK expression was evaluated by real-time PCR and immunohistochemistry. For OTM, a nickel-titanium closed-coil spring was fixed between the upper incisors and upper-left first molar to move the first molar in the mesial direction in wild-type (WT) and TNFR1/TNFR2-deficient (TNFRsKO) mice. After OTM, the number of RANK-positive cells on the compression side was evaluated by immunohistochemistry. Results: RANK levels were enhanced in TNF-α-treated osteoclast precursors in vitro . RANK mRNA expression levels and the number of RANK-positive cells were higher in TNF-α-injected mice than in phosphate-buffered saline-injected mice. RANK-positive cells increased on the compression side of the alveolar bone in WT mice because of the mechanical loading. In addition, the number of RANK-positive cells on the compression side was significantly higher in WT mice than in TNFRsKO mice after OTM. Conclusion: These results suggest that TNF-α induces RANK expression in vitro and at baseline in vivo, as well as on the compression side during OTM.