Design and optimization of bioreactor to boost carbon dioxide assimilation in RuBisCo-equipped Escherichia coli. (October 2020)

• Record Type: Journal Article
• Authors: Tan, Shih-I; Ng, I-Son
• Is Part Of: Bioresource technology. Volume 314(2020)
• Publication Date: 2020-10
• Subjects: RuBisCo -- E. coli -- Bioreactor -- CO2 assimilation -- Chaperone; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2020.123785
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: A simple mass balance to assess the CO2 assimilation capability was established. An evaluation of bioreactor effect on CO2 assimilation capability was reported. Forced diffusion of CO2 provides better performance on CO2 assimilation. Addition of GroELS chaperone dramatically enhanced CO2 assimilation performance. RuBisCo-based pathway was found to alter the proteins and morphology of E. coli . Abstract: Global warming is a surging issue that has provoked the demand of green process to mitigate carbon dioxide. In this context, RuBisCo-equipped Escherichia coli has first developed and evaluated the CO2 -assimiliable capability based on the mass balance in three devices: Flask-based in CO2 incubator (FIC), two-layered device (TLD) and CO2 bubbling device (CBD) systematically. With the forced diffusion of 5% CO2 in CBD, which confers an efficient attack of CO2 to RuBisCo, the CO2 assimilation increased from −5.03 to −2.63 g-CO2 /g-DCW. Furthermore, boosted CO2 assimilation ability was observed by co-expression of GroELS chaperone with 71% reduction on CO2 release. By DNA sequencing and tandem MS/MS analysis, the toxicity of RuBisCo and PRK was identified to interfere the sugar metabolism and energy producing, while the cell morphology was changed and observed in RuBisCo-equipped E. coli . Our study provides a new perspective of higher CO2 assimilation for sustainable to eco-friendly green bioprocess.