Automated and rapid identification of multidrug resistant Escherichia coli against the lead drugs of acylureidopenicillins, cephalosporins, and fluoroquinolones using specific Raman marker bands. Issue 8 (18th June 2020)

• Record Type: Journal Article
• Authors: Götz, Theresa; Dahms, Marcel; Kirchhoff, Johanna; Beleites, Claudia; Glaser, Uwe; Bohnert, Jürgen A.; Pletz, Mathias W.; Popp, Jürgen; Schlattmann, Peter; Neugebauer, Ute
• Is Part Of: Journal of biophotonics. Volume 13:Issue 8(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-06-18
• Subjects: antibiotic susceptibility testing -- Escherichia coli -- multidrug‐resistant Gram‐negative bacteria -- Raman spectroscopy -- weighted sum score; Photonics -- Periodicals; Optical materials -- Periodicals; Optics -- Periodicals; Medical instruments and apparatus -- Periodicals; 621.3605
• DOI: 10.1002/jbio.202000149
• Languages: English
• ISSNs: 1864-063X

Abstract

Abstract: A Raman‐based, strain‐independent, semi‐automated method is presented that allows the rapid (<3 hours) determination of antibiotic susceptibility of bacterial pathogens isolated from clinical samples. Applying a priori knowledge about the mode of action of the respective antibiotic, we identified characteristic Raman marker bands in the spectrum and calculated batch‐wise weighted sum scores from standardized Raman intensity differences between spectra of antibiotic exposed and nonexposed samples of the same strains. The lead substances for three relevant antibiotic classes (fluoroquinolone ciprofloxacin, third‐generation cephalosporin cefotaxime, ureidopenicillin piperacillin) against multidrug‐resistant Gram‐negative bacteria (MRGN) revealed a high sensitivity and specificity for the susceptibility testing of two Escherichia coli laboratory strains and 12 clinical isolates. The method benefits from the parallel incubation of control and treated samples, which reduces the variance due to alterations in cultivation conditions and the standardization of differences between batches leading to long‐term comparability of Raman measurements. Abstract : The global spread of multidrug resistant pathogens is a major threat for public health and requires scientific advances in three critical areas: antimicrobial discovery, rapid diagnostics, and infection prevention and antimicrobial stewardship. This paper presents a powerful approach for rapid diagnostics. Standard clinical microbiological techniques, which include antibiotic susceptibility testing assays in automated machines after time‐consuming bacterial culture, usually yield results only after one or 2 days. The presented Raman‐based, strain‐independent, semi‐automated method allows the rapid (<3 hours) determination of antibiotic susceptibility of bacterial pathogens isolated from clinical samples.