Survival of Curtobacterium flaccumfaciens pv. flaccumfaciens in weeds. (25th May 2020)

• Record Type: Journal Article
• Authors: Nascimento, Daniele M.; Oliveira, Letícia R.; Melo, Luana L.; Silva, João C.; Soman, José M.; Girotto, Karine T.; Eburneo, Renan P.; Ribeiro‐Junior, Marcos R.; Sartori, Maria M. P.; Silva Júnior, Tadeu A. F.; Maringoni, Antonio C.
• Is Part Of: Plant pathology. Volume 69:Number 7(2020)
• Page Start: 1357
• Page End: 1367
• Publication Date: 2020-05-25
• Subjects: bacterial wilt -- common bean -- disease management -- ecology -- phytobacteria -- principal component analysis; Agricultural pests -- Periodicals; Plant diseases -- Periodicals; 632
• DOI: 10.1111/ppa.13206
• Languages: English
• ISSNs: 0032-0862

Abstract

Abstract: Weeds are important alternative hosts of pathogens, responsible for the survival and spread of phytopathogenic bacteria. Our study evaluated the potential of weeds as hosts of Curtobacterium flaccumfaciens pv. flaccumfaciens (Cff), causal agent of bacterial wilt, one of the main diseases of common beans. Cff survival was evaluated in the phyllosphere and in the rhizosphere of 21 weeds, in four experiments under field conditions, during the years 2018 and 2019. The aerial part of the plant was inoculated by spraying bacterial suspension (10 7 cfu/ml) of Cff, while the soil of the growing pots was infested with the same suspension. Cff survival was evaluated every 7 days, for 70 days. The identity of the bacterium was confirmed by PCR with the specific primers CffFOR2 and CffREV4, from strains recovered from all samples. Principal component analysis (PCA) showed that high temperatures and rainfall reduced Cff survival in the phyllosphere, while high temperatures reduced the survival of the bacterium in the rhizosphere. Our results demonstrated that Amaranthus viridis (family Amaranthaceae), Conyza bonariensis, Emilia fosbergii, Galinsoga parviflora, Gnaphalium purpureum (Asteraceae), Raphanus sativus, Lepidium virginicum (Brassicaceae), Commelina benghalensis (Commelinaceae), Ipomoea triloba (Convolvulaceae), Cyperus rotundus (Cyperaceae), Senna obtusifolia (Fabaceae), Digitaria insularis (Poaceae), Nicandra physalodes, and Solanum americanum (Solanaceae) are potential hosts for Cff. Their eradication in common bean fields is recommended, especially in fields with a history of bacterial wilt occurrence. Abstract : Curtobacterium flaccumfaciens pv. flaccumfaciens, causal agent of bacterial wilt in beans, can survive in the weeds phyllosphere and rhizosphere. Weeds can be considered as potential hosts and therefore possible inoculum sources.