Synthesis of Injectable Shear‐Thinning Biomaterials of Various Compositions of Gelatin and Synthetic Silicate Nanoplatelet. Issue 8 (16th March 2020)

• Record Type: Journal Article
• Authors: Xue, Chengbin; Xie, Huifang; Eichenbaum, James; Chen, Yi; Wang, Yonggang; van den Dolder, Floor W.; Lee, Junmin; Lee, KangJu; Zhang, Shiming; Sun, Wujin; Sheikhi, Amir; Ahadian, Samad; Ashammakhi, Nureddin; Dokmeci, Mehmet R.; Kim, Han‐Jun; Khademhosseini, Ali
• Is Part Of: Biotechnology journal. Volume 15:Issue 8(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-03-16
• Subjects: gelatin -- injectability -- laponite -- rheology -- shear‐thinning biomaterial; Biotechnology -- Periodicals; 660.605
• DOI: 10.1002/biot.201900456
• Languages: English
• ISSNs: 1860-6768

Abstract

Abstract: Injectable shear‐thinning biomaterials (iSTBs) have great potential for in situ tissue regeneration through minimally invasive therapeutics. Previously, an iSTB was developed by combining gelatin with synthetic silicate nanoplatelets (SNPs) for potential application to hemostasis and endovascular embolization. Hence, iSTBs are synthesized by varying compositions of gelatin and SNPs to navigate their material, mechanical, rheological, and bioactive properties. All compositions (each component percentage; 1.5–4.5%/total solid ranges; 3–9%) tested are injectable through both 5 Fr general catheter and 2.4 Fr microcatheter by manual pressure. In the results, an increase in gelatin contents causes decrease in swellability, increase in freeze‐dried hydrogel scaffold porosity, increase in degradability and injection force during iSTB fabrication. Meanwhile, the amount of SNPs in composite hydrogels is mainly required to decrease degradability and increase shear thinning properties of iSTB. Finally, in vitro and in vivo biocompatibility tests show that the 1.5–4.5% range gelatin–SNP iSTBs are not toxic to the cells and animals. All results demonstrate that the iSTB can be modulated with specific properties for unmet clinical needs. Understanding of mechanical and biological consequences of the changing gelatin–SNP ratios through this study will shed light on the biomedical applications of iSTB on specific diseases. Abstract : Injectable shear‐thinning biomaterials (iSTBs) have great potential for in situ tissue regeneration through minimally invasive therapeutics. Here, mechanical, rheological, and biological properties of gelatin and silicate nanoplatelet (SNP) composite hydrogels are described. Through this work, the understanding of mechanical and biological consequences by changing gelatin–SNP ratios will shed light on the biomedical applications of iSTB on specific diseases.