Id1 expression in kidney endothelial cells protects against diabetes‐induced microvascular injury. Issue 8 (26th June 2020)

• Record Type: Journal Article
• Authors: Sharma, Shree; Plotkin, Matthew
• Is Part Of: FEBS open bio. Volume 10:Issue 8(2020)
• Page Start: 1447
• Page End: 1462
• Publication Date: 2020-06-26
• Subjects: diabetes -- endothelial cell -- Id1 -- microvascular injury -- senescence; Molecular biology -- Periodicals; Cytology -- Periodicals; Life sciences -- Periodicals; Biological Science Disciplines -- Periodicals; Molecular Biology -- Periodicals; Cell Biology -- Periodicals; Cytology; Life sciences; Molecular biology; Periodicals; 572.805
• DOI: 10.1002/2211-5463.12793
• Languages: English
• ISSNs: 2211-5463

Abstract

Abstract : The inhibitor of differentiation (Id) transcription regulators, which are induced in response to oxidative stress, promote cell proliferation and inhibit senescence. Inhibitor of differentiation 1 (Id1) expression is limited to endothelial cells (EC) in the normal mouse kidney and is required for a normal response to injury. Endothelial dysfunction leads to the development of diabetic nephropathy, and so, we hypothesized that endothelial Id1 may help protect against hyperglycemia‐induced microvascular injury and nephropathy. Here, we tested this hypothesis by using streptozotocin to induce diabetes in Id1 knockout (KO) mice and WT B6;129 littermates and examining the mice at 3 months. Expression of Id1 was observed to be increased 15‐fold in WT kidney EC, and Id1 KO mice exhibited increased mesangial and myofibroblast proliferation, matrix deposition, and albuminuria compared with WT mice. Electron microscopy demonstrated peritubular capillary EC injury and lumen narrowing, and fluorescence microangiography showed a 45% reduction in capillary perfusion area with no reduction in CD31‐stained areas in Id1 KO mice. Microarray analysis of EC isolated from WT and KO control and diabetic mice demonstrated activation of senescence pathways in KO cells. Kidneys from KO diabetic mice showed increased histological expression of senescence markers. In addition, premature senescence in cultured KO EC was also seen in response to oxidative stress. In conclusion, endothelial Id1 upregulation with hyperglycemia protects against microvascular injury and senescence and subsequent nephropathy. Abstract : The inhibitor of differentiation (Id) transcription regulators are induced in response to oxidative stress and inhibit cell senescence. Id1 is expressed in kidney endothelial cells, and levels are increased in response to hyperglycemia. Using streptozotocin‐induced hyperglycemia in Id1 knockout mice, we demonstrate that Id1 protects against microvascular injury and endothelial cell senescence and subsequent nephropathy.