Statistical analysis of the crystallinity index of nanocellulose produced from Kraft pulp via controlled enzymatic hydrolysis. (23rd January 2020)

• Record Type: Journal Article
• Authors: Ribeiro, Ruan Stevan Almeida; Bojorge, Ninoska; Pereira, Nei
• Is Part Of: Biotechnology and applied biochemistry. Volume 67:Number 3(2020)
• Page Start: 366
• Page End: 374
• Publication Date: 2020-01-23
• Subjects: CCRD -- endoglucanase -- enzymatic hydrolysis -- kraft pulp -- nanocellulose; Biotechnology -- Periodicals; Biochemical engineering -- Periodicals; Biochemistry -- Periodicals; Biochemistry -- Periodicals; Genetic Techniques -- Periodicals; Microbiological Techniques -- Periodicals; 660.6
• DOI: 10.1002/bab.1873
• Languages: English
• ISSNs: 0885-4513

Abstract

Abstract: Enzymatic hydrolysis processes can change the physical characteristics of nanocellulose derived from Kraft pulp. Among these attributes are its crystallinity index and dimensions. In this study, we determined the optimal conditions under which nanocellulose could be produced enzymatically with the greatest increase of the crystallinity index relative to its initial state. Application of Central Composite Rotatable Design statistical analysis to the experiments was employed to direct an increase the crystallinity index in 10% at the 24‐H hydrolysis time. Upon establishment of ideal levels of starting material and enzyme, reactions were carried out at hydrolysis times of 24, 48, and 72 H under these ideal parameters. The effectiveness of deagglomeration was demonstrated by measuring the hydrodynamic diameter of the particles by dynamic light scattering. Scanning electron microscopy was performed on four samples, the original material, kraft pulp, and hydrolyzed biomaterials at 72 H in the ideal parameters. The hydrolyzed material with the best statistical data, revealing a fiber diameter of 180 nm, disclosing to be biomaterial with nanocellulose dimensions. Abstract : Controlled enzymatic hydrolysis treatments for nanocelulose production with the highest crystallinity indexes of hydrolyzed Kraft pulp. Apparent hydrodynamic diameter as function of enzymatic hydrolysis time, and confirmed by SEM.