Fluorescence microscopy‐based analysis of apoptosis induced by platinum nanoparticles against breast cancer cells. (31st May 2020)

• Record Type: Journal Article
• Authors: Puja, Patel; Vinita, Nadar Manimaran; Devan, Umapathy; Velangani, Antony Joseph; Srinivasan, Pappu; Yuvakkumar, Rathinam; Arul Prakash, Pitchan; Kumar, Ponnuchamy
• Is Part Of: Applied organometallic chemistry. Volume 34:Number 9(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-05-31
• Subjects: apoptosis -- breast cancer cells -- fluorescence microscope -- mitochondrial membrane potential -- platinum nanoparticles; Organometallic chemistry -- Periodicals; Organometallic compounds -- Periodicals; 547.05
• DOI: 10.1002/aoc.5740
• Languages: English
• ISSNs: 0268-2605

Abstract

Abstract : In recent years, capping molecules onto the surface of nanomaterials has become an interesting field of research. This idea facilitates the biological applications of nanomaterials with a modified surface. Keeping this in mind, the present study addresses the development of polymeric platinum nanoparticles using polyvinyl pyrrolidone (PVP). High‐throughput characterization indicates that polymeric platinum nanoparticles have an excellent surface morphology and good dispersity in aqueous solution. More specifically, high resolution‐transmission electron microscopy studies showed that the polymeric platinum nanoparticles were spherical and measured 2–10 nm. Furthermore, the polymeric platinum nanoparticles were evaluated for anticancer properties against human MCF‐7 breast cancer cell lines. The results show that polymeric platinum nanoparticles inhibited the growth of cancer cells in a dose‐dependent manner with a half‐maximum inhibitory concentration of 96.36 μg ml −1 . In addition, fluorescence‐based staining methods confirmed an inquest in the pattern of cell death inferring late apoptotic bodies, nuclear fragmentation, mitochondrial membrane potential and generation of reactive oxygen species. The overall findings suggest that the polymeric platinum nanoparticles confer anticancer activity and may be suitable chemotherapeutic agents in the future. Finally, the results from this study can be extended to other types of cancer as well. Abstract : In the recent past, the platinum based nanoparticles gain much attention among researchers. However, the adverse side‐effects of platinum nanoparticles upon treatment limits its usage. Keeping this in mind, we demonstrate a facile method in developing platinum nanoparticles which involves a reaction between chloroplatinic acid and polyvinyl pyrrolidone in the presence of ethanol targeting breast cancer cell lines.