In vitro antimicrobial effects and mechanism of air plasma‐activated water on Staphylococcus aureus biofilm. Issue 8 (29th March 2020)

• Record Type: Journal Article
• Authors: Xu, Zimu; Zhou, Xiaoxia; Yang, Weishu; Zhang, Yudi; Ye, Zhengxin; Hu, Shuheng; Ye, Chaobin; Li, Yunxia; Lan, Yan; Shen, Jie; Ye, Xiaodong; Yang, Fan; Cheng, Cheng
• Is Part Of: Plasma processes and polymers. Volume 17:Issue 8(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-03-29
• Subjects: bacteria inactivation -- biofilm regrowth capacity -- plasma‐activated water (PAW) -- reactive oxygen species (ROS) -- Staphylococcus aureus (S. aureus) biofilm; Plasma polymerization -- Periodicals; Plasma-enhanced chemical vapor deposition -- Periodicals; Plasma chemistry -- Periodicals
• DOI: 10.1002/ppap.201900270
• Languages: English
• ISSNs: 1612-8850

Abstract

Abstract: The deactivation effects and mechanism of plasma‐activated water (PAW) against Staphylococcus aureus biofilm and the inhibitory effect on the biofilm regrowth capacity for the surviving S. aureus post PAW treatment were investigated in vitro. Systematic measurements on bacterial cultivability, metabolic capacity, membrane integrity and intracellular reactive oxygen species (ROS) concentration under two different experimental categories were carried out after PAW treatment. Considerable deactivation effects were discovered on biofilm S. aureus on both prolonging the PAW inducement or treatment time. The rising concentration of intracellular ROS and the PAW‐contained RS might exert synergistic effects in deactivating the biofilm S. aureus . Moreover, the biomass, bacterial cultivability and metabolic capacity of the regrown S. aureus biofilm all significantly declined along with the increasing PAW inducement or treatment time. Therefore, except for the short‐term rapid inactivation effects on the biofilm, the PAW treatment could also exert long‐term inhibitory effects on the regeneration of S. aureus biofilm. Abstract : This study investigates the deactivation effect and mechanism of air plasma‐activated water (PAW) against a Staphylococcus aureus biofilm, as well as the inhibitory effect on the biofilm regrowth capacity. Systematic measurements on bacterial cultivability, metabolic capacity, membrane integrity, and intracellular reactive oxygen species (ROS) level were carried out after PAW treatment. Experimental results show that PAW treatment could considerably exert both a short‐term rapid inactivation effect and long‐term growth‐inhibitory impact on the S. aureus biofilm. The PAW‐contained reactive species and the enhanced concentration of intracellular ROS post‐treatment might have resulted in a synergistic effect in the biofilm deactivation process.