Exilibacterium tricleocarpae gen. nov., sp. nov., a marine bacterium from coralline algae Tricleocarpa sp. Issue 5 (4th May 2020)

• Record Type: Journal Article
• Authors: Wang, Guanghua; Zheng, Xinfeng; Xu, Shuailiang; Dang, Ge; Su, Hongfei; Liao, Zhiheng; Chen, Biao; Huang, Wen; Liang, Jiayuan; Yu, Kefu
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 5(2020)
• Page Start: 3427
• Page End: 3432
• Publication Date: 2020-05-04
• Subjects: Exilibacterium tricleocarpae -- phylogenetics -- phylogenomics; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.004189
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-stain-negative, non-spore-forming, aerobic, curved rod-shaped bacterium, designed strain R142 T, was isolated from a coralline algae Tricleocarpa sp. in the Beibu Gulf, China. Optimal growth occurred with 0–0.5 % (w/v) NaCl, at 25 °C and at pH 8. Global alignment based on 16S rRNA gene sequences indicated that strain R142 T shared 93.8 % similarity with its closest type strain, Pseudomaricurvus alkylphenolicus KU14G T . Phylogenetic analyses showed that strain R142 T forms a distinct branch alongside Maricurvus nonylphenolicus KU41E T, Pseudoteredinibacter isoporae SW-11 T, Pseudomaricurvus alkylphenolicus KU14G T, Pseudomaricurvus alcaniphilus MEBiC06469 T and Aestuariicella hydrocarbonica SM-6 T . The major polar lipids of strain R142 T were phosphatidylethanolamine and phosphatidylglycerol. The primary cellular fatty acids were C16 : 0, C16 : 1 ω7 c, C18 : 1 ω7 c, C18 : 0 and C14 : 0 . The genome DNA G+C ratio was 56.4 mol%. The only detected respiratory quinone was ubiquinone 8. The low 16S rRNA gene sequence similarity and differences in cellular fatty acids readily distinguished strain R142 T from all validly published type strains. Strain R142 T is therefore suggested to represent a novel species of a new genus, for which the name Exilibacterium tricleocarpae gen. nov., sp. nov. is proposed. The type strain of Exilibacterium tricleocarpae is R142 T (=MCCC 1K03816 T =KCTC 72138 T ).