Pseudoalteromonas rhizosphaerae sp. nov., a novel plant growth-promoting bacterium with potential use in phytoremediation. Issue 5 (28th April 2020)

• Record Type: Journal Article
• Authors: Navarro-Torre, Salvadora; Carro, Lorena; Rodríguez-Llorente, Ignacio D.; Pajuelo, Eloísa; Caviedes, Miguel Ángel; Igual, José Mariano; Klenk, Hans-Peter; Montero-Calasanz, Maria del Carmen
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 5(2020)
• Page Start: 3287
• Page End: 3294
• Publication Date: 2020-04-28
• Subjects: Arthrocnemum macrostachyum -- heavy metals -- odiel marshes -- rhizosphere -- nitrogen fixation; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.004167
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : Strain RA15 T was isolated from the rhizosphere of the halophyte plant Arthrocnemum macrostachyum growing in the Odiel marshes (Huelva, Spain). RA15 T cells were Gram stain-negative, non-spore-forming, aerobic rods and formed cream-coloured, opaque, mucoid, viscous, convex, irregular colonies with an undulate margin. Optimal growth conditions were observed on tryptic soy agar (TSA) plates supplemented with 2.5 % NaCl (w/v) at pH 7.0 and 28 °C, although it was able to grow at 4–32 °C and at pH values of 5.0–9.0. The NaCl tolerance range was from 0 to 15 %. The major respiratory quinone was Q8 but Q9 was also present. The most abundant fatty acids were summed feature 3 (C16 : 1 ω7 c and/or C16 : 1 ω6 c ), C17 : 1 ω 8 c and C16 : 0 . The polar lipids profile comprised phosphatidylglycerol and phosphatidylethanolamine as the most abundant representatives. Phylogenetic analyses confirmed the well-supported affiliation of strain RA15 T within the genus Pseudoalteromonas, close to the type strains of Pseudoalteromonas neustonica, Pseudoalteromonas prydzensis and Pseudoalteromonas mariniglutinosa . Results of comparative phylogenetic and phenotypic studies between strain RA15 T and its closest related species suggest that RA15 T could be a new representative of the genus Pseudoalteromonas, for which the name Pseudoalteromonas rhizosphaerae sp. nov. is proposed. The type strain is RA15 T (=CECT 9079 T =LMG 29860 T ). The whole genome has 5.3 Mb and the G+C content is 40.4 mol%.