Cellulomonas endophytica sp. nov., isolated from Gastrodia elata Blume. Issue 5 (2nd April 2020)

• Record Type: Journal Article
• Authors: Li, Yan-Qiong; Zhang, Hui; Xiao, Min; Dong, Zhou-Yan; Zhang, Jing-Yi; Narsing Rao, Manik Prabhu; Li, Wen-Jun
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 5(2020)
• Page Start: 3091
• Page End: 3095
• Publication Date: 2020-04-02
• Subjects: Cellulomonas endophytica -- endophyte -- Gastrodia elataBlume -- genome analysis; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.004133
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-stain-positive, facultatively anaerobic and non-motile strain, designated SYSUP0004 T, was isolated from the tubers of Gastrodia elata Blume collected from Yunnan Province, PR China. The 16S rRNA gene sequence result showed that the strain SYSUP0004 T shared low similarity (97.7 %) with the type strain of Cellulomonas marina . SYSUP0004 T grew at pH 6.0–9.0 (optimum, pH 8.0), temperature 4–30 °C (optimum, 28 °C) and could tolerate NaCl up to 4 % w/v (optimum in the absence of NaCl). The cell-wall peptidoglycan type was A4 β with an interpeptide bridge l -ornithine–d -glutamic acid. Cell-wall sugars were mannose, ribose, glucose, galactose and fucose. The menaquinone was MK-9(H4 ). The major fatty acids were anteiso-C15:0, anteiso-C15 : 1 A, C16 : 0 and anteiso-C17 : 0 . The polar lipids of SYSUP0004 T were diphosphatidylglycerol, unidentified phosphoglycolipid, phosphatidylinositol mannosides and unidentified glycolipid. The genomic DNA G+C content was 76.5 %. The average nucleotide identity values between SYSUP0004 T and members of the genus Cellulomonas were below the cut-off level (95–96 %) recommended as the ANI criterion for interspecies identity. Thus, based on the above results strain SYSUP0004 T represents a novel species of the genus Cellulomonas, for which the name Cellulomonas endophytica sp. nov. is proposed. The type strain, SYSUP0004 T (=KCTC 49025 T =CGMCC 1.16405 T ).