A case of canine cutaneous pythiosis in Thailand. Issue 4 (14th February 2020)

• Record Type: Journal Article
• Authors: Chindamporn, Ariya; Kammarnjessadakul, Patcharee; Kesdangsakonwut, Sawang; Banlunara, Wijit
• Is Part Of: Access microbiology. Volume 2:Issue 4(2020)
• Publication Date: 2020-02-14
• Subjects: dog -- skin -- phylogenetic analysis -- Pythium insidiosum -- Thailand; Microbiology -- Periodicals; 579
• DOI: 10.1099/acmi.0.000109
• Languages: English
• ISSNs: 2516-8290

Abstract

Abstract : Introduction. Pythium insidiosum causes pythiosis in humans and animals in tropical and subtropical climates. The clinical manifestations in humans are mostly systemic, vascular or ocular forms, in contrast to animals, which are cutaneous, subcutaneous and gastrointestinal forms. The highest incidence of human cases is reported in Thailand, however, no canine pythiosis has been documented yet. Case presentation. A female, mixed-breed, stray dog showed severe extensive ulcerative haemorrhagic dermatitis at the perineum involving the anus and tail. On cytology, there were sparse branching septate fungal hyphae. The tissue samples were subjected to polymerase chain reaction and gene sequencing for fungal identification. Conclusion. The results of the internal transcribed spacer 1 and 2 (ITS1 and ITS2) gene had 99 % homology to Pythium insidiosum (accession no. FJ17396) and the COX2 gene (accession no. GQ451572). The phylogenetic tree of both genes was classified in clade ATH. This is the first fully documented diagnosis of canine cutaneous pythiosis in Thailand.