Rapid and Efficient Genetic Transformation of Sorghum via Agrobacterium‐Mediated Method. Issue 4 (12th October 2018)

• Record Type: Journal Article
• Authors: Do, Phat Tien; Lee, Hyeyoung; Nelson‐Vasilchik, Kimberly; Kausch, Albert; Zhang, Zhanyuan J.
• Editors: Birchler, James; Ecker, Joseph; Martin, Cathie R.; Stitt, Mark; Zhou, Jian-Min
• Other Names: Stacey Gary editorInChief.
• Is Part Of: Current protocols in plant biology. Volume 3:Issue 4(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-10-12
• Subjects: Agrobacterium‐mediated -- genetic transformation -- P898012 -- sorghum -- standard binary vector; Botany -- Laboratory manuals; Plants; Botany; Botany; Laboratory Manual; Periodical; Laboratory manuals; 580.28
• DOI: 10.1002/cppb.20077
• Languages: English
• ISSNs: 2379-8068

Abstract

Abstract: Genetic transformation via Agrobacterium ‐mediated methodology has been used in many sorghum studies. However, the transformation efficiency still varies significantly due to high dependence on sorghum genotypes and technical expertise. In this article, we describe a sorghum transformation procedure in sufficient detail using a public genotype, P898012. This system utilizes a standard binary transgenic vector carrying the bar gene as a selectable marker and immature embryos as starting explants. Glufosinate is employed as the selective agent during callus and shoot induction. This procedure is relatively rapid, efficient, highly reproducible, and should be applicable for many other sorghum genotypes. © 2018 by John Wiley & Sons, Inc.