Recombinant Viral Vectors as Neuroscience Tools. Issue 1 (22nd March 2019)

• Record Type: Journal Article
• Authors: Chen, Shih‐Heng; Haam, Juhee; Walker, Mitzie; Scappini, Erica; Naughton, John; Martin, Negin P.
• Editors: Crawley, Jacqueline N.; Gerfen, Charles R.; Rogawski, Michael A.; Sibley, David R.; Skolnick, Phil; Wray, Susan
• Is Part Of: Current protocols in neuroscience. Issue 87:Issue 1(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-03-22
• Subjects: AAV -- gene delivery -- lentivirus -- rabies delta‐G -- retrovirus -- virus; Neurosciences -- Laboratory manuals; Neurosciences; Cytological Techniques; Histological Techniques; Nervous System -- anatomy & histology; Neurosciences; Laboratory Manual; Laboratory manuals; 612.8
• DOI: 10.1002/cpns.67
• Languages: English
• ISSNs: 1934-8576

Abstract

Abstract: Recombinant viruses are highly efficient vehicles for in vivo gene delivery. Viral vectors expand the neurobiology toolbox to include direct and rapid anterograde, retrograde, and trans‐synaptic delivery of tracers, sensors, and actuators to the mammalian brain. Each viral type offers unique advantages and limitations. To establish strategies for selecting a suitable viral type, this article aims to provide readers with an overview of viral recombinant technology, viral structure, tropism, and differences between serotypes and pseudotypes for three of the most commonly used vectors in neurobiology research: adeno‐associated viruses, retro/lentiviruses, and glycoprotein‐deleted rabies viruses. © 2019 by John Wiley & Sons, Inc.