Inhibitory effects of Lentinus edodes mycelia polysaccharide on α-glucosidase, glycation activity and high glucose-induced cell damage. (15th October 2020)

• Record Type: Journal Article
• Authors: Cao, Xiangyu; Xia, Ying; Liu, Dan; He, Yonglin; Mu, Teng; Huo, Yapeng; Liu, Jianli
• Is Part Of: Carbohydrate polymers. Volume 246(2020)
• Publication Date: 2020-10-15
• Subjects: LMP Lentinus edodes mycelia polysaccharide -- AGEs advanced glycation end products -- HUVECs human umbilical vein endothelial cells -- ROS reactive oxygen species -- MAPK mitogen-activated protein kinase -- WHO World Health Organization -- IDF International Diabetes Federation -- pNPG p-nitrophenyl-α-D-glucopyranoside -- BSA bovine serum albumin -- MTT thiazolyl blue tetrazolium bromide -- DMSO dimethyl sulfoxide -- FBS fetal bovine serum -- SD standard deviation -- ANOVA analysis of variance -- Glu glucose -- ERKs Extracellular signal-regulated kinases -- JNKs c-Jun N-terminal kinases; p-nitrophenyl-α-D-glucopyranoside (PubChem CID: 259380) -- Glucose (PubChem CID: 5793) -- Sodium carbonate (PubChem CID: 10340) -- p-nitrophenol (PubChem CID: 980) -- Penicillin (PubChem CID: 5904) -- Streptomycin (PubChem CID: 19649) -- Thiazolyl blue tetrazolium bromide (PubChem CID: 64965) -- Dimethyl sulfoxide (PubChem CID: 679) -- DCFH-DA (PubChem CID: 104913) -- Hoechst 33258 (PubChem CID: 135705211); Lentinus edodes mycelia polysaccharide -- α-glucosidase -- Advanced glycation end products -- Oxidative stress -- Apoptosis; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2020.116659
• Languages: English
• ISSNs: 0144-8617

Abstract

Graphical abstract: Lentinus edodes mycelia polysaccharide (LMP) showed a reversible inhibition on α-glucosidase activity in a mixed-type manner. LMP inhibited AGEs formation effectively. In addition, LMP alleviated high glucose-induced oxidative stress and apoptosis in HUVECs by inhibiting the MAPK signaling pathways. Highlights: LMP showed a reversible inhibition on α-glucosidase activity in a mixed-type manner. LMP had remarkable capacity to inhibit the formation of AGEs in glucose-BSA model. LMP alleviated the oxidative stress induced by high glucose in HUVECs significantly. LMP is a potential agent for diabetes and related complications via multiple targets. Abstract: At present, diabetes and diabetic complications have become one of the serious diseases affecting human health. In this study, the inhibitory effects of Lentinus edodes mycelia polysaccharide (LMP) on α-glucosidase activity, the formation of advanced glycation end products (AGEs) and high glucose-induced human umbilical vein endothelial cells (HUVECs) damage were explored. The interaction between LMP and α-glucosidase and the inhibition against AGEs formation were investigated with spectroscopic techniques. The results revealed that LMP had a reversible inhibition on α-glucosidase activity in a mixed-type manner. When the concentration of LMP was 2.7 mM, the inhibition rate was 34.38 %. LMP quenched the fluorescence of α-glucosidase through the static quenching and formed the LMP-α-glucosidase complex. At 310 K, the number of binding sites ( n ) and binding constant ( Kb ) were 1.01 and 3.71 × 10 4 L mol −1, respectively. In addition, LMP could inhibit the formation of AGEs. Compared with 40 mM glucose treatment group, treatment with 0.05 mM LMP for 48 h increased the cell viability from 70.17% to 91.14% and decreased ROS production from 3.33-fold to 1.21-fold. LMP inhibited high glucose-induced activation of MAPK signaling pathways. These findings may promote the application of LMP in the functional food industry.