Effect of lyophilization on the bacterial cellulose produced by different Komagataeibacter strains to adsorb epicatechin. (15th October 2020)

• Record Type: Journal Article
• Authors: Chen, Si-Qian; Cao, Xiao; Li, Zhaofeng; Zhu, Jie; Li, Lin
• Is Part Of: Carbohydrate polymers. Volume 246(2020)
• Publication Date: 2020-10-15
• Subjects: Bacterial cellulose -- Komagataeibacter -- Polyphenol -- Epicatechin -- Mechanics -- Ionic liquid; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2020.116632
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Bacterial strains affect the mechanical properties and epicatechin binding of BC. Lyophilization affects the structure of BC from different strains to various extent. BC produced by strain ATCC 53582 adsorbed less epicatechin than 23767 and 700178. The structure of BC produced by ATCC 53582 collapsed in freeze-drying. The estimated DP of BC produced by ATCC 53582 was higher than 23767 and 700178. Abstract: Bacterial cellulose (BC) has been widely used as a model system to investigate the interaction of polyphenols with the polysaccharides of cell walls. In this study, the water absorption ability and the adsorption ability of epicatechin of the never-dried and freeze-dried BC produced by a high-yield Komagataeibacter hansenii strain ATCC 53582 was compared with two normal-yield strains. The structural characteristics of BC were investigated via microscopy observation and mechanical/rheological tests. The 1-butyl-3-methylimidazolium acetate/dimethyl sulfoxide ([BMIM]Ac/DMSO) co-solvent was used to dissolve BC to calculate the degree of polymerization (DP). Results showed that compared with the other two strain, the BC synthesised by ATCC 53582 had a higher cellulose concentration (1.2 wt%) but lower epicatechin adsorption (29 μg/mg under 4 mM, pH 7). Its fibril network collapsed and led to a reduced recovery ratio (86 %) in the compression-relaxation test, which may be due to large DP (2856).