Isolation, structural elucidation, and immunoregulation properties of an arabinofuranan from the rinds of Garcinia mangostana. (15th October 2020)

• Record Type: Journal Article
• Authors: Zhang, Shaojie; Li, Zhengguo; Wang, Xuelian; An, Lijun; Bao, Jiahe; Zhang, Jie; Cui, Jianlin; Li, Yuhao; Jin, Da-Qing; Tuerhong, Muhetaer; Abudukeremu, Munira; Ohizumi, Yasushi; Xu, Jing; Guo, Yuanqiang
• Is Part Of: Carbohydrate polymers. Volume 246(2020)
• Publication Date: 2020-10-15
• Subjects: Sodium chloride (PubChem CID: 5234) -- Trifluoroacetic acid (PubChem CID: 6422) -- Dimethyl sulfoxide (PubChem CID: 679) -- Methyl iodide (PubChem CID: 6328) -- Sodium borohydride (PubChem CID: 4311764) -- Tricaine (PubChem CID: 261501) -- Streptomycin (PubChem CID: 19649) -- Penicillin (PubChem CID: 2349) -- 3-amino, 4-aminomethyl-2′, 7′-difluorescein diacetate (PubChem CID: 2762646) -- 2', 7'-dichlorofluorescein diacetate (PubChem CID: 104913); Garcinia mangostana L. -- Polysaccharide -- NMR -- GC–MS -- Conformational analysis -- Macrophages -- Zebrafish embryo -- Immunoregulation; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2020.116567
• Languages: English
• ISSNs: 0144-8617

Abstract

Graphical abstract: Highlights: An arabinofuranan (GMP90-1) was first isolated from the rinds of G. mangostana . GMP90-1 was composed of arabinose, galactose, and rhamnose. GMP90-1 presented a rigid rod structure rather than a triple-helical structure. GMP90-1 stimulated RAW264.7 cells to produce TNF- α, IL-6, IL-1 β, and NO. The immunoenhancing activity of GMP90-1 was confirmed by an in vivo zebrafish model. Abstract: In our search for bioactive polysaccharides as immunomodulatory agents, an arabinofuranan (GMP90-1) was purified and characterized from the rinds of Garcinia mangostana L. GMP90-1 (absolute molecular weight: 5.30 × 10 3 g/mol) was found to be composed of arabinose, galactose, and rhamnose. The backbone of GMP90-1 was determined as (1→5)-linked α -l -Ara f, (1→2, 3, 5)-linked α -l -Ara f, (1→3, 5)-linked α -l -Ara f, (1→6)-linked β -d -Gal p, and (1→2)-linked α -l -Rha p . Conformational analysis revealed GMP90-1 to exist as a rigid rod structure in sodium chloride solution. To explore its potential as immunomodulatory agents, an in vitro cell screening was performed and GMP90-1 was found to significantly enhance the phagocytic uptake of neutral red and improve the secreted level of nitric oxide (NO), interleukin (IL)-6, IL-1 β, and tumor necrosis factor- α (TNF- α ) of macrophages. Furthermore, the cellular immunomodulatory activities were confirmed by the in vivo zebrafish experiment, which suggested that GMP90-1 with immunomodulatory effects could be considered as a potential immunomodulatory for immune diseases.