Immunoactive polysaccharides produced by heterotrophic mutant of green microalga Parachlorella kessleri HY1 (Chlorellaceae). (15th October 2020)

• Record Type: Journal Article
• Authors: Sushytskyi, Leonid; Lukáč, Pavol; Synytsya, Andriy; Bleha, Roman; Rajsiglová, Lenka; Capek, Peter; Pohl, Radek; Vannucci, Luca; Čopíková, Jana; Kaštánek, Petr
• Is Part Of: Carbohydrate polymers. Volume 246(2020)
• Publication Date: 2020-10-15
• Subjects: Chlorella -- Rhamnans -- Xylogalactofuranan -- Structure -- Aggregation -- Immunomodulatory activity; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2020.116588
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Three polysaccharide fractions were obtained from hot water extract of Parachlorella kessleri HY1 biomass. The fractions were defined as non-sulphated heteropolysaccharides containing rhamnose, xylose and galactose at various ratios. These polysaccharides tended to self-segregate in an aqueous solution upon cooling. Polysaccharides also demonstrated strong immunoactive properties testing in vitro on normal and melanoma immune cells. Abstract: Hot water extract from biomass of heterotrophic mutant green alga Parachlorella kessleri HY1 (Chlorellaceae) was deproteinised, and three polysaccharidic fractions were obtained by preparative chromatography. The low-molecular fraction (1.5 × 10 4 g mol −1 ) was defined mainly as branched O -2-β-xylo-(1→3)-β-galactofuranan where xylose is partially methylated at O -4. Two high-molecular fractions (3.05 × 10 5 and 9.84 × 10 4 g mol −1 ) were complex polysaccharides containing α-l -rhamnan and xylogalactofuranan parts in different ratios. The polysaccharides were well soluble in hot water and, upon cooling, tended to self-segregate. Immunomodulatory activities of the obtained fractions were preliminary tested using ELISA, FACS and ImmunoSpot kits. The polysaccharides increased the TNF-α production in melanoma bearing mice with much higher intensity than in healthy mice. This was in agreement with the FACS results on T and B cells indicating their possibly secondary activation by innate immunity cells.