Evaluation of the expression of bone marrow‐derived mesenchymal stem cells and cancer‐associated fibroblasts in the stroma of gastric cancer tissue. Issue 4 (27th May 2020)

• Record Type: Journal Article
• Authors: Minami, Taizan; Aoyagi, Keishiro; Kawahara, Akihiko; Murakami, Naotaka; Isobe, Taro; Tanaka, Yuya; Kaku, Hideaki; Fujita, Fumihiko; Akagi, Yoshito
• Is Part Of: Annals of gastroenterological surgery. Volume 4:Issue 4(2020)
• Page Start: 464
• Page End: 474
• Publication Date: 2020-05-27
• Subjects: BM‐MSC -- CAF -- gastric cancer -- immunohistochemistry; Digestive organs -- Surgery -- Periodicals; 617.43
• DOI: 10.1002/ags3.12347
• Languages: English
• ISSNs: 2475-0328

Abstract

Abstract: Aim: Cancer‐associated fibroblasts (CAFs) generated by bone marrow‐derived mesenchymal stem cells (BM‐MSCs) play an important role in cancer progression. In this study, we investigated the relationships of BM‐MSCs and CAFs in resected gastric cancers with the clinicopathological factors of patients. Methods: We analyzed 120 gastric cancer patients who underwent gastrectomy. Immunostaining was performed with an anti‐CD271 antibody (BM‐MSCs) and anti‐α‐smooth muscle actin (αSMA) antibody (CAFs). Staining intensity was used to divide patients into low and high expression groups. Observation sites in cancer tissues were invasive, central, and whole portions. Results: Expression of αSMA was significantly related to depth of tumor invasion (T), lymph node metastasis (N), lymphatic invasion (ly), venous invasion (v), and stage. Expression of CD271 was significantly related to v, stage, stromal volume, and tumor infiltration pattern (INF). Overall survival (OS) of the high expression group was significantly lower than that of the low expression group for both αSMA and CD271. Multivariate analysis showed that N, αSMA (whole), and CD271 (invasive) were independent prognostic factors. Conclusions: Cancer‐associated fibroblasts and BM‐MSCs are related to the progression, invasion, and prognosis of gastric cancer and may be therapeutic targets of gastric cancer. Abstract : We immunohistochemically examined bone marrow‐derived mesenchymal stem cells and cancer‐associated fibroblasts in gastric cancer tissues and obtained results indicating that they were associated with tumor infiltration and stroma, and closely related to prognosis.